Topic
Molecular breeding
About: Molecular breeding is a research topic. Over the lifetime, 2120 publications have been published within this topic receiving 56908 citations.
Papers published on a yearly basis
Papers
More filters
01 Jan 2015
TL;DR: The aim of this research is to establish a baseline for the application of DNA markers in diversity analysis, and to demonstrate the importance of these markers in the development of transgenic animals.
Abstract: Part 1: Sorghum: Introduction.- 1. Sorghum: Origin, classification, biology and improvement.- Part 2: Advances in DNA marker research.- 2. DNA markers in diversity analysis.- 3. Linkage mapping.- 4. Application of DNA markers for genetic improvement.- 5. Heterosis prediction using DNA markers.- Part 3: Advances in genomics research.- 6. Genomics and bioinformatics resources.- 7. Post-genome sequencing developments.- 8. Molecular marker development using bioinformatic tools.- Part 4: Advances in transgenic research .- 9. Advances in genetic transformation.- 10. Genetic engineering for novel traits.
19 citations
01 Jan 2005
19 citations
TL;DR: The data generated in this study allowed the survey of genetic variability in a heterogeneous and scantily examined germplasm, with repercussions on its exploitation in breeding programs.
Abstract: Onion is an economically important crop cultivated worldwide since ancient times. Over the centuries, domestication and outbreeding have had a significant influence on its genetic pool, leading to a high degree of biodiversity. In this study, using kompetitive allele-specific PCR (KASP) genotyping technology, we explored the genetic variation of 73 onion accessions (including wild species, commercial, and local varieties) from different areas of the world. The SNP dataset inspection returned 375 polymorphic loci with a very low percentage of non-calling sites (0.03%). Eight-nine percent of the onions amplified all polymorphic loci and were considered for a population structure analysis. The ΔK method suggested four populations and enabled the identification of genepools, reflecting the geographical origin of the samples. Through statistical studies, our SNP set has proven to be successful, revealing population-specific alleles and potential candidates for use in future breeding programs. Notably, 74 loci were associated with phenotypic traits (bulbing photoperiod, bulb shape, or bulb color), and 3 loci were identified as putative targets of selection associated with onion improvement. Fifty-three pairs of SNPs were co-inherited, and among them, 17 were both trait-associated and in linkage disequilibrium. In conclusion, the data generated in this study allowed the survey of genetic variability in a heterogeneous and scantily examined germplasm, with repercussions on its exploitation in breeding programs.
19 citations