Topic
Molecular breeding
About: Molecular breeding is a research topic. Over the lifetime, 2120 publications have been published within this topic receiving 56908 citations.
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18 May 2018
TL;DR: The indel molecular marker SEQ7593 is located on a tenth chromosome of a Chinese pumpkin, and is 280bp in size as mentioned in this paper, which can be directly used for creating an assistant breeding system of photoperiod insensitivity character molecular markers.
Abstract: The invention discloses an indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of the indel molecular marker, and belongs to the technical field of molecule detection. The indel molecular marker SEQ7593 is located on a tenth chromosome of a Chinese pumpkin, and is 280bp in size. The nucleotide sequence of the indel molecular marker is shown as SEQ ID NO.1.The indel molecular marker SEQ7593 can be directly used for creating an assistant breeding system of photoperiod insensitivity character molecular markers. Primer amplification designed according to the indel molecular marker can be applied to assistant breeding of pumpkin breed improvement molecules, technical support is provided for pumpkin photoperiod insensitivity molecular breeding, and timefor conventional gene positioning is shortened greatly.
2 citations
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22 Jul 2015
TL;DR: In this paper, molecular markers for haplotype identification of a paddy rice CMS restoring gene Rf-1 segment and applications thereof are provided for the fields of plant biology and molecular breeding.
Abstract: The invention provides molecular markers for haplotype identification of a paddy rice CMS restoring gene Rf-1 segment and applications thereof, and belongs to the fields of plant biology and molecular breeding. By comparing various types of restorer parent sequencing data of paddy rice, 32 sites of restoring gene Rf-1 segment polymorphism are screened, and a plurality of primer pairs and TaqMan-MGB probe compositions are designed. Sequences of the TaqMan-MGB probe compositions are respectively shown as SEQID NO.1-128. The 32 primer pairs and the TaqMan-MGB probe compositions are further prepared into an OpenArray chip. Haplotype identification of the paddy rice CMS restoring gene Rf-1 segment can be achieved by utilization of the molecular markers or the chip. The molecular markers can be applied for haplotype identification of the CMS restoring gene Rf-1 segment in the paddy rice resource and for breeding of three-line hybrid rice.
2 citations
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2 citations