Topic
Molecular breeding
About: Molecular breeding is a research topic. Over the lifetime, 2120 publications have been published within this topic receiving 56908 citations.
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TL;DR: This chapter describes Agrobacterium tumefaciens-mediated transformation protocols for lowland tetraploid switchgrass cultivar Alamo, one of the most important bioenergy crops for lignocellulose ethanol production.
Abstract: Switchgrass (Panicum virgatum L.) is one of the most important bioenergy crops for lignocellulose ethanol production. Molecular breeding provides a powerful tool to supplement conventional switchgrass breeding by introducing or editing genes of interest. In this chapter, we describe Agrobacterium tumefaciens-mediated transformation protocols for lowland tetraploid switchgrass cultivar Alamo.
2 citations
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02 Apr 2021
2 citations
01 Jan 2006
2 citations
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TL;DR: This review presents the development and application of genetic transformation in sweet potato.
Abstract: Sweet potato is an important root crop for food production world-wide and serves as raw material for the processing of feeds,starches and bioethanol in various industriesEstablishment of efficient genetic transformation system in sweet potato not only provides a platform for functional verification of important genes,but also is an essential tool for molecular breeding in sweet potato improvementThis review presents the development and application of genetic transformation in sweet potato
2 citations
01 Jan 2003
TL;DR: In this article, the authors describe two examples of introgression of QTLs into elite material undertaken in the framework of an upland rice breeding program, targeting root depth and osmotic adjustment.
Abstract: Water deficit is an increasingly important constraint limiting rice production, highlighting the need for varieties with improved drought tolerance and technologies that increase water-use efficiency. Drought tolerance, however, is a complex character resulting from the interaction of many quantitative component traits. Marker-based genetic studies permitted the identification of reliable QTLs for root traits and osmotic adjustment. To demonstrate the value of marker-aided selection to manipulate QTLs, we describe two examples of introgression of QTLs into elite material undertaken in the framework of an upland rice breeding program. The first study targeted root depth. Several QTLs from Azucena, a deep-rooted variety, were introgressed into IR64 through three cycles of backcrosses with selection based on marker information alone. The evaluation of the BC3 F3 families showed what progress could be expected and how progress was affected by lack of precision in QTL positions. For the second study, targeting osmotic adjustment, we used the advanced backcross QTL analysis method, which allows the simultaneous discovery and transfer of interesting QTLs, a procedure that appeared more adapted to a breeding program. We transferred QTLs for osmotic adjustment from IR62266, an indica donor, into IR60080-46A, a japonica elite line, conducted QTL analysis in a BC3F3 population, and outlined a strategy to construct improved near-isogenic lines. The candidate gene approach was used to associate genes with QTLs in this population. Association studies based on linkage disequilibrium in breeders' working collections could allow further progress by locating QTLs more precisely. (Resume d'auteur)
2 citations