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Molecular breeding

About: Molecular breeding is a research topic. Over the lifetime, 2120 publications have been published within this topic receiving 56908 citations.


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Dissertation
01 Apr 2017
TL;DR: This research establishes molecular breeding capability for adapting common bean (Phaseolus vulgaris L.) to UK growing conditions using genotyping-by-sequencing data and identifies five high-probability candidate determinants of the broad virulence of Psph race 6, including avirulence protein AvrD.
Abstract: This research establishes molecular breeding capability for adapting common bean (Phaseolus vulgaris L.) to UK growing conditions. A high-resolution linkage map was constructed for a bi-parental recombinant inbred population (large brown x small white haricot) using genotyping-by-sequencing data. Pre-breeding material was exploited to enable genetic mapping and marker-assisted selection of essential adaptive traits, including (1) resistance to halo blight, caused by Pseudomonas syringae pathovar phaseolicola (Psph), (2) root architecture related to abiotic stress tolerance and nutrient acquisition, (3) earliness of maturity, (4) plant architecture amenable to mechanical harvest, and (5) seed coat colour of consumer interest. A 500-kb mapping interval was defined for quantitative resistance to the broadly virulent Psph race 6, a devastating bacterial pathogen that threatens global bean production with losses from halo blight. Complementary research generating high-quality draft genomes for 32 pathogenically and geographically diverse isolates of Psph identified five high-probability candidate determinants of the broad virulence of Psph race 6, including avirulence protein AvrD. Pathogenicity effectors that are highly conserved within the pathovar were identified as candidate targets for potential race-nonspecific resistance to halo blight. Putative QTL for root architecture traits associated with water and nutrient acquisition were detected on chromosome Pv07. A useful breeding strategy may be to select for larger taproot diameter in view of the comparatively high heritability of this trait. Potentially desirable alleles on Pv07 are linked in coupling phase with the dominant allele of seed coat pigmentation factor P. Identification of lines recombinant for these alleles may prove useful for the introgression of genes governing physiological resilience into white-seeded varieties adapted to UK growing conditions. Provisional QTL for morphological and reproductive traits of agronomic importance, including plant architecture, growth stage and yield, were identified using phenotypic data obtained from pilot field and polytunnel evaluations of the recombinant inbred population.

1 citations

Patent
19 Jul 2019
TL;DR: In this article, a method of constructing a rice molecular marker map based on competitive allelic PCR and an application in rice molecular breeding thereof, and belongs to the technical field of agriculture.
Abstract: The invention provides a method of constructing a rice molecular marker map based on competitive allelic PCR and an application in rice molecular breeding thereof, and belongs to the technical field of agriculture. The method aims to search for SNP sites by whole gene recombination sequencing of rice and mining SNP data of recombination sequencing; 565 SNP sites needed for breeding are obtained through combining existing SNP databases based on the SNP sites; the selected 565 SNP sites are utilized to perform molecular marking, and the rice molecular marker map is constructed. Physical locationof SNP markers of the rice molecular marker map constructed by the method is clear, detection method is simple. The rice molecular marker map can be directly used for partial molecular identificationand genetic background analysis of important agronomic traits, and has important application value to improve the efficiency of the rice molecular breeding.

1 citations

Patent
27 Oct 2017
TL;DR: In this paper, a molecular marker relevant to cotton fiber intensity from upland cotton and application thereof was proposed. But this marker was used only for the mark auxiliary selection of the fiber intensity.
Abstract: The invention relates to the field of cotton molecular breeding, in particular to a molecular marker relevant to cotton fiber intensity from upland cotton and application thereof. The molecular marker comprises CGR6078, DPL0041 and BNL1707, and is used for the mark auxiliary selection of the cotton fiber intensity.

1 citations

01 Dec 2011
TL;DR: In this paper, the authors discuss two fundamental differences between classical genetic improvement (CGI) or breeding and genetic engineering improvement (GEI); the latter is an extension of the first but is more accurate so, it gives greater safety to the consumer; and transformation by genetic engineering is equivalent to a natural mutation.
Abstract: This essay discusses two statements: a) there are no fundamental differences between classical genetic improvement (CGI) or breeding and genetic engineering improvement (GEI); the latter is an extension of the first but is more accurate so, it gives greater safety to the consumer; and b) transformation by genetic engineering is equivalent to a natural mutation. CGI and the GEI pursue objectives that can be complementary; but its foundations, methods and biological implications are different: the CGI works within the limits of sexual compatibility, using the species genetic diversity as a favorable source of DNA, which gives the precision and recombination mechanisms of sexual reproduction. The CGI uses foreign DNA integrated into a transgenic chimera, which is inserted imprecisely known in the chromosomal space of the transforming and generates a different locus in each independent transgenic event. Such dispersion can lead to an accumulation of transgenic chimeras in their progeny, and possible deleterious effects. By excluding the quantitative phenotypic characteristics as yield, GEI depends on the progress made by the CGI to

1 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202383
2022153
2021156
2020143
2019169
2018137