Molecular breeding

About: Molecular breeding is a research topic. Over the lifetime, 2120 publications have been published within this topic receiving 56908 citations.

Reverse molecular breeding method using corn hybrid germplasm

Abstract: The invention relates to the field of molecular breeding, and particularly relates to a reverse molecular breeding method using corn hybrid germplasm. The reverse molecular breeding method comprises the steps of (1), deriving high-density genotypes of a hybrid male parent and a hybrid female parent from high-density genotypes of an embryo and a seed coat; (2), planting a maize hybrid of F1 generation in a greenhouse, bagging and selfing to generate an F2 seed; (3), extracting leaf DNA (Deoxyribose Nucleic Acid) from a corn F2 colony; (4), measuring a genotype of SNP (Single Nucleotide Polymorphism) of the F2 colony by virtue of low-density gene chips on 192 SNP loci of corns; (5), selecting a plant the genotype of which has highest similarity with the female parent and the male parent, bagging and selfing to generate an F3 seed; and (6), planting and selfing the obtained seed in the greenhouse, and establishing a selfing line which is similar to the hybrid male parent and the hybrid female parent. Compared with a conventional hybrid second cycle line breeding method, the reverse molecular breeding method has the characteristics of short period (1-2 years), fixed heterotic pattern, high beneficial gene utilization rate, and the like.

Regulatory Paradigms for Modern Breeding

Abstract: Modern breeding, based upon molecular biology using genetic information, has made rapid advances. Breeders using rDNA techniques properly can think of this technique as traditional biotechnology. Within the past ten years, breeders have begun to use newer techniques [site-directed nuclease techniques (SDNs), RNAi, and synthetic biology] to create and to develop plants and animals with desired genetic traits. In this chapter printed in the NABC 26 Report (May 2015), the authors address the question: What is the appropriate regulatory paradigm for modern breeding?

Advance on Molecular Breeding of Rice Bacterial Blight Resistance

Abstract: Rice bacterial blight is one of the major bacterial disease.Rice bacterial blight resistance molecular breeding includes:the identification and cloning of resistance genes,molecular marker assisted selection,gene engineering breeding and creation and improvement of resistance gene resource.Until now 22 resistance genes have been identified.Two of the resistance genes,such as Xal and Xa21,which have been cloned by positional cloning.With the development of molecular biology research on resistance genes from Xal to xa24(t),rice bacterial blight resitance molecular breeding will realize the directional operation of gene resource and application of breeding.

SNP molecular marker linked to effective tiller number QTL of wheat and application thereof

Abstract: The invention relates to the field of wheat molecular breeding, and specifically discloses an SNP molecular marker linked to an effective tiller number QTL QPTN. Sau-4B of wheat and application thereof. The molecular marker is KASP-1, and can be obtained by amplification of primers of which nucleotide sequences are shown in SEQ ID NO. 1-3. Detection analysis shows that the molecular marker can beused for accurately tracking the effective tiller number QTL QPTN. Sau-4B of the wheat, predicting the effective tiller number characteristics of the wheat, and further facilitating molecular design breeding. The invention also discloses a molecular marker method for identifying the effective tiller number QTL QPTN. Sau-4B of the wheat, and by adopting the method, the accuracy of prediction of theeffective tiller number can be improved so as to quickly screen out wheat varieties or lines with increased effective tiller number for breeding, and the breeding process of high-yield varieties of the wheat can be greatly speeded up.

Microsatellite marker and its application in the study of swine genetics and breeding

Abstract: In this paper,the application of microsatellite marker was reviewed in the study of swine genetics and breeding, for instance, construction of genetic lindage map, marker assistal Selection,mapping of quantitative trait loci (QTL ), evaluation of genetic diversity and parentage testing etc..Microsatellite marder has many advantages such as a great quantity, extensive dispersion around the genome, high polyomorphism, Mendelian codominant inheritance, rapid and convenient detection,and exhibits fine application prospects in the field of genetics study and breeding .