Molecular breeding

About: Molecular breeding is a research topic. Over the lifetime, 2120 publications have been published within this topic receiving 56908 citations.

Molecular marker related to porcine reproduction traits based on PGR genes and detection method and application of molecular marker

Abstract: The invention discloses a molecular marker related to porcine reproduction traits based on PGR genes and a detection method and application of the molecular marker. The molecular marker is a porcine PGR gene segment, the porcine PGR gene segment includes a polymorphic site, and the polymorphic site is, for example, the 1319th in a sequence shown by SEQ ID NO.1, and has variations of C/T basic groups. The detection method comprises the steps that a primer is designed according to a nucleotide sequence of the molecular marker; PCR amplification is carried out with a genome DNA of a pig to be detected serving as a template, and the type of the polymorphic site in a PCR amplification product is judged. The application of the molecular marker in porcine reproduction trait selection is further disclosed. The molecular marker related to the porcine reproduction traits based on the PGR genes and the detection method and application of the molecular marker are provided according to a PGR gene mutation site, and a foundation is provided for porcine molecular breeding and study of porcine reproduction physiological mechanisms.

Analysis and synthesis in animal breeding and genetics

Abstract: 1.は じめに 家畜育種における分析 と総合 と題 して、以下に意見 を述べ る。 はじめに、本論の内容の概略を示す。 2.本 論の題 にある 「家 畜育種」とは何 か?一 般 に 「分析 と総合」とは何か?に ついて触れる。 3.家 畜育種 には 「総合が不可欠である」ことについ て、い くつかの局面か ら述べる。 4.家 畜育種 における総合的な概念の活用 につ いて、 “育種価”を例 として示す 。 5.総 合 に必要 な、要素間の交互作用 について述べ る。 6.総 合的な観点が不可 欠な“複雑系 ”の科学につい て述べる。 7.総 合 的な側面 を持つ、家畜育種 に関する研究 を二 つ紹介する。 8.エ ネルギーの利用効率を例 として、動物の総合的 な進化 について述べる。 9.今 後、重要 になると考えられる概念 について提案 する。 10.家 畜育種 における分析 と総合についてまとめる。

Snp combination for analyzing diversity of chinese cabbage germplasm resource and for molecular breeding, and use thereof

Abstract: Disclosed are a SNP combination for analyzing the genetic diversity of Chinese cabbage and for molecular breeding, and a use thereof The use provided by the present invention in particular is the use of 571 SNP sites or a substance for detecting the 571 SNP sites in any one of the following: (1) construction of a Chinese cabbage DNA fingerprint database; (2) genetic diversity analysis and/or evolution analysis of the Chinese cabbage germplasm resource; and (3) molecular breeding of the Chinese cabbage The physical location of the 571 SNP sites is determined based on the whole genome sequence alignment of the Chinese cabbage variety Chiifu-402-2, and the version number of the whole genome sequence of the Chinese cabbage variety Chiifu-402-2 is V15; and the 571 SNP sites are BrSNPA01001-BrSNPA10043 Experiments have demonstrated that these 571 SNP sites can be used for genetic diversity analysis and evolution analysis of the Chinese cabbage; moreover, these 571 SNP sites can also be used for molecular breeding of the Chinese cabbage, and provide important technical support therefor

Special SNP codominant molecular marker primer in rice blast resistant gene of rice

Abstract: The invention discloses a special SNP codominant molecular marker primer in rice blast resistant gene of rice, and belongs to the field of plant molecular breeding. Sequences of the marker primer are separately as follows: Pi35-PF:GCGTACAGCGTTCGCTCTATA; Pi35-PR:CTTCAAAAGCTCACAACCA; Pi35-NR: CTGCAAAACAAAGCAAACGTG; Pi35-NF:CCGTCCTCCCTCCAGCATATATGTATAAGG. The primer is utilized for carrying out PCR amplification on rice DNA, and can be used for quickly judging the gene type of to-be-detected rice Pi35. The method is simple, quick, low in cost, capable of being widely applied to molecular marker assisted selective breeding or rice genetic resource Pi35 gene type identifying.

Using the Knowledge of Post-transcriptional Regulations to Guide Gene Selections for Molecular Breeding in Soybean

Abstract: The omics approaches allow the scientific community to successfully identify genomic regions associated with traits of interest for marker-assisted breeding. Agronomic traits such as seed color, yield, growth habit, and stress tolerance have been the targets for soybean molecular breeding. Genes governing these traits often undergo post-transcriptional modifications, which should be taken into consideration when choosing elite genes for molecular breeding. Post-transcriptional regulations of genes include transcript regulations, protein modifications, and even the regulation of the translational machinery. Transcript regulations involve elements such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) for the maintenance of transcript stability or regulation of translation efficiency. Protein modifications involve molecular modifications of target proteins and the alterations of their interacting partners. Regulations of the translational machinery include those on translation factors and the ribosomal protein complex. Post-transcriptional regulations usually involve a set of genes instead of a single gene. Such a property may facilitate molecular breeding. In this review, we will discuss the post-transcriptional modifications of genes related to favorable agronomic traits such as stress tolerance, growth, and nutrient uptake, using examples from soybean as well as other crops. The examples from other crops may guide the selection of genes for marker-assisted breeding in soybean.