Topic
Multiplex polymerase chain reaction
About: Multiplex polymerase chain reaction is a research topic. Over the lifetime, 6409 publications have been published within this topic receiving 221244 citations.
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TL;DR: A new PCR-based method has been developed to monitor inoculated wine fermentations based on the variation in the number and position of introns in the mitochondrial gene COX1, which allows early detection of the starter yeast strain throughout the fermentation process.
64 citations
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TL;DR: This is the first report to genetically identify and detect Rhizopus species from human mucormycosis specimens, and may expand the possibility of this multiplex PCR system not only to identify isolated fungi, but also as a screening method for visceral mucormYcosis.
64 citations
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TL;DR: The relative efficiency (compared to performing separate assays for each virus) and apparent sensitivity of mPCR suggest its potential application for routine molecular diagnostic purposes.
64 citations
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TL;DR: An event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity using multiplex-PCR together with oligonucleotide microarray.
Abstract: With the increasing development of genetically modified organism (GMO) detection techniques, the polymerase chain reaction (PCR) technique has been the mainstay for GMO detection An oligonucleotide microarray is a glass chip to the surface of which an array of oligonucleotides was fixed as spots, each containing numerous copies of a sequence-specific probe that is complementary to a gene of interest So it is used to detect ten or more targets synchronously In this research, an event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity using multiplex-PCR together with oligonucleotide microarray A commercial GM soybean (GTS 40-3-2) and six GM maize events (MON810, MON863, Bt176, Bt11, GA21, and T25) were detected by this method The results indicate that it is a suitable method for the identification of these GM soybean and maizes
64 citations
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TL;DR: The use of ethidium monoazide (EMA) to eliminate contaminating DNA in a polymerase chain reaction is described to retain the retention of the highly sensitive nature of PCR with the ability to amplify template DNA at concentrations lower than those of contaminatingDNA.
64 citations