scispace - formally typeset
Search or ask a question
Topic

Multiplex polymerase chain reaction

About: Multiplex polymerase chain reaction is a research topic. Over the lifetime, 6409 publications have been published within this topic receiving 221244 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: A new PCR-based method has been developed to monitor inoculated wine fermentations based on the variation in the number and position of introns in the mitochondrial gene COX1, which allows early detection of the starter yeast strain throughout the fermentation process.

64 citations

Journal ArticleDOI
TL;DR: This is the first report to genetically identify and detect Rhizopus species from human mucormycosis specimens, and may expand the possibility of this multiplex PCR system not only to identify isolated fungi, but also as a screening method for visceral mucormYcosis.

64 citations

Journal ArticleDOI
TL;DR: The relative efficiency (compared to performing separate assays for each virus) and apparent sensitivity of mPCR suggest its potential application for routine molecular diagnostic purposes.

64 citations

Journal ArticleDOI
Jia Xu1, Shuifang Zhu1, Haizhen Miao1, Wensheng Huang1, Minyan Qiu1, Yan Huang1, Xuping Fu1, Yao Li1 
TL;DR: An event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity using multiplex-PCR together with oligonucleotide microarray.
Abstract: With the increasing development of genetically modified organism (GMO) detection techniques, the polymerase chain reaction (PCR) technique has been the mainstay for GMO detection An oligonucleotide microarray is a glass chip to the surface of which an array of oligonucleotides was fixed as spots, each containing numerous copies of a sequence-specific probe that is complementary to a gene of interest So it is used to detect ten or more targets synchronously In this research, an event-specific detection strategy based on the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene is being developed for its high specificity using multiplex-PCR together with oligonucleotide microarray A commercial GM soybean (GTS 40-3-2) and six GM maize events (MON810, MON863, Bt176, Bt11, GA21, and T25) were detected by this method The results indicate that it is a suitable method for the identification of these GM soybean and maizes

64 citations

Journal ArticleDOI
TL;DR: The use of ethidium monoazide (EMA) to eliminate contaminating DNA in a polymerase chain reaction is described to retain the retention of the highly sensitive nature of PCR with the ability to amplify template DNA at concentrations lower than those of contaminatingDNA.

64 citations


Network Information
Related Topics (5)
Locus (genetics)
42.7K papers, 2M citations
75% related
Gene
211.7K papers, 10.3M citations
70% related
Antigen
170.2K papers, 6.9M citations
68% related
Plasmid
44.3K papers, 1.9M citations
68% related
Complementary DNA
55.3K papers, 2.7M citations
67% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023275
2022448
2021172
2020176
2019221
2018220