Topic
Multiplex polymerase chain reaction
About: Multiplex polymerase chain reaction is a research topic. Over the lifetime, 6409 publications have been published within this topic receiving 221244 citations.
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TL;DR: Two multiplex PCR methods were developed to screen large collections of trimethoprim-resistant Escherichia coli isolates for the most prevalent resistance determinants and proved to be sensitive and specific for detection of the five selected genes.
58 citations
TL;DR: A DNA fragment from Mycobacterium tuberculosis is cloned and sequenced for use in the identification of members of the M. tuberculosis complex and a polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of theM.
Abstract: We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.
58 citations
TL;DR: The method was simultaneously extended for detection of HTST pasteurized milk samples and cheeses of bovine and buffalo origin and showed a high degree of specificity.
58 citations
TL;DR: This is the first report on the molecular characterization of E. coli diarrheogenic isolates in Colombia and the potential role in childhood diarrhea in this geographic area, and most of them belonged to the phylogenetic groups A and B1, known to be associated with intestinal pathogens.
Abstract: Acute diarrheal disease is a leading cause of childhood morbidity and mortality in the developing world and Escherichia coli intestinal pathogens are important causative agents Information on the epidemiology of E coli intestinal pathogens and their association with diarrheal disease is limited because no diagnostic testing is available in countries with limited resources To evaluate the prevalence of E coli intestinal pathogens in a Caribbean–Colombian region, E coli clinical isolates from children with diarrhea were analyzed by a recently reported two-reaction multiplex polymerase chain reaction (Gomez-Duarte et al, Diagn Microbiol Infect Dis 2009;63:1–9) The phylogenetic group from all E coli isolates was also typed by a single-reaction multiplex polymerase chain reaction We found that among 139 E coli strains analyzed, 20 (144%) corresponded to E coli diarrheagenic pathotypes Enterotoxigenic, shiga-toxin–producing, enteroaggregative, diffuse adherent, and enteropathogenic E coli
58 citations
TL;DR: The multiplex PCR-LDR-UA approach developed provides for an accurate, inexpensive, and high-throughput assay that does not exhibit false positive or false negative signals, thus making it highly suitable for animal genotyping.
58 citations