Topic
Multiplex polymerase chain reaction
About: Multiplex polymerase chain reaction is a research topic. Over the lifetime, 6409 publications have been published within this topic receiving 221244 citations.
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TL;DR: This study compares 4 different multiplex PCR assays for the recovery of common respiratory viruses using the xTAG respiratory viral panel fast, Fast-track Respiratory Pathogen assay, Easyplex respiratory pathogen 12 kit, and an in-house multiplex real-time PCR assay.
55 citations
TL;DR: A multiplex allele-specific polymerase chain reaction (MAS-PCR) that detects the most commonly observed isoniazid, rifampin, and ethambutol resistance-associated mutations in a single assay provides a rapid, potentially more cost-effective, method of detecting multidrug-resistant TB.
55 citations
TL;DR: New sets of primers for PCR identification of the common Mediterranean α‐globin gene rearrangements, and established reaction conditions that provide easily interpretable, unambiguous diagnoses, should facilitate carrier screening and identification of couples at risk for α‐thalassemia.
Abstract: The most frequent molecular lesions causing alpha-thalassemia are deletions of one or more alpha-globin genes. Detection of these deletions generally requires genomic Southern analysis, which is cumbersome and time consuming. We have designed new sets of primers for PCR identification of the common Mediterranean alpha-globin gene rearrangements, including the -alpha3.7 deletion and the alphaalphaalpha(anti3.7) triplication, the -alpha4.2 deletion, and the --Med allele. We have established reaction conditions that provide easily interpretable, unambiguous diagnoses. Some of the PCR reactions are multiplex, simultaneously identifying several genotypes, thus reducing the time and cost of screening and prenatal testing. The use of these methods should facilitate carrier screening and identification of couples at risk for alpha-thalassemia.
55 citations
TL;DR: A very sensitive and new real-time multiplex PCR method for the quantification of genetically modified (GM) maize crops in food materials was developed and validated for an ABI Prism 7700 Sequence Detection System.
Abstract: A very sensitive and new real-time multiplex PCR method for the quantification of genetically modified (GM) maize crops in food materials was developed and validated for an ABI Prism 7700 Sequence Detection System. In the assay described, fluorescence-labelled TaqMan probes were chosen to detect the amplified DNA fragments during PCR. In this multiplex approach, maize-specific DNA (zein) and 35S-CaMV promoter-specific DNA fragments are amplified in the same tube. The method was tested for the detection and quantification of the four maize events that are approved in Europe and contain the 35S-CaMV promoter: Bt11, Bt176, Mon810 and T25 maize. Quantification was based on a standard curve prepared from certified maize flour reference material prepared by the Institute for Reference Materials and Measurements. Quantification within the range of the standard curve (0.05–1% GM maize) and up to 100% was possible. Repeatability of the method for each GM maize event was determined; coefficients of variations ranged from 28–40%. In addition, three internal Nestle laboratories successfully applied this method and comparable results were obtained.
55 citations
TL;DR: A sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV 71 and CV-A16 and should prove useful for clinical diagnosis of EV71 or CV- a16 infections.
Abstract: The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.
55 citations