Showing papers on "Murashige and Skoog medium published in 1976"

Tissue culture propagation of tropical foliage plants

Abstract: Procedures were established for clonal multiplication in vitro ofCordyline terminalis Kunth,Dracaena godseffiana Hort.,Scindapsus aureus Engler, andSyngonium podophyllum Schott. Shoot tips of actively growing terminals were selected as explants forCordyline andDracaena, and lateral buds were employed forScindapsus andSyngonium. The basal nutrient medium contained Murashige and Skoog salts, 3% sucrose, 100 mg per li-inositol, and 0.4 mg per l thiamine · HCl. The optima with respect to auxin, cytokinin, adenine sulfate · 2H2O, and NaH2PO4 · H2O addenda were determined. Also assessed were the influences of certain physical qualities of the nutrient medium and of the light intensity of the culture environment. The multiplication of each of the four plants was achieved by repeatedly subculturing the shoots that arose in vitro. Rates of plant increase per year per explant were calculated conservatively to be as follows:Syngonium, 5,000;Scindapsus, 100,000;Dracena, 300,000; andCordyline, 500,000.

In vitro plant regeneration from leaf expiants of Lycopersicon esculentum (tomato)

Abstract: Leaf discs from 15 mutant clones of tomato were tested for their morphogenetic response in Murashige and Skoog medium supplemented with 12 combinations of the growth regulators napthaleneacetic aci...

Studies on Vegetative Propagation of Tulip

Abstract: The present investigation aimed at the development of a new technique for propagation of tulip bulb by means of tissue culture in vitro. Excised scales of the bulbs derived from eight commercial varieties; i. e., ‘Apeldoorn’, ‘Cesar Frank’, ‘Cramoisi Brilliant’, ‘Defiance’, ‘Fuga’, ‘Monsieur S. Mottet’, ‘Red Emperor’, ‘William Pitt’and three botanical species, namely, Tulipa hageri, T. praestans and T. tubergeniana‘Emir’which were cultured on synthetic media for the purpose of determining the most favorable conditions for their organ formation.1) Callus formation of cultured scales obtained from‘Apeldoorn’, ‘Red Emperor’, ‘Defiance’and T. praestans was spontaneously induced on the modified Murashige and Skoog medium supplemented with α-naphthaleneacetic acid (NAA) or 2, 4-dichlorophenoxyacetic acid (2•4-D) at the appropriate concentration, whereas the scales of‘William Pitt’and‘Cramoisi Brilliant’failed to develop the callus in any case.2) With regard to the formation of shoot-like protuberances on the cultured scales, the scale tissue of‘Apeldoorn’cultured on the medium containing either NAA 5mg/l plus kinetin 1mg/l or 2•4-D 1mg/l plus kinetin 1mg/l was capable of producing the shoot like protuberances. In the case of T. hageri, the protuberances were formed on a medium with NAA 2, 10 and 25mg/l to each of which was added 1mg/l kinetin.3) The scales of‘Apeldoorn’, ‘William Pitt’and‘Fuga’formed adventitious roots 20 to 30 weeks after inoculation, when they were cultured on a medium supplemented with NAA or a suitable combination of NAA and kinetin.

Effect of auxin-cytokinin interaction on organogenesis in haploid callus of Pelargonium hortorum.

Abstract: Bud differentiation from haploid anther callus of geranium was achieved on Murashige and Skoog medium supplemented with 0.5 mg per 1 NAA and 2.5 mg per 1 kinetin. At concentrations higher than 5 mg per 1 kinetin, malformation and tissue senescence were evident.

Effects of kinetin and gibberellin a3 on callus growth and organ formation inLimnophila chinensis tissue culture

Abstract: The action of exogenously applied hormones in the induction of morphogenesis inLimnophila chinensis (Osb.) Merr. tissue culture has been demonstrated. Stem expiants were grown on Murashige and Skoog’s medium containing various levels of kinetin, gibberellic acid and indole-3-acetic acid. Formation of roots, shoots (normal or abnormal), plantlets and friable, hard or nodulated calluses depended largely on the hormone levels used. The formation of normal shoots and roots were stimulated by treatment with kinetin. GA3 treatment stimulated the bud differentiation but inhibited the root initiation. A combination of kinetin and GA3 gave variable results.

Cell wall regeneration and colony formation from isolated single geranium protoplasts in microculture

Abstract: Geranium (Pelargonium hortorum Bailey) protoplasts were obtained from haploid callus tissue from anther cultures after 3 h of incubation with 2% cellulase (Onozuka SS) and 0.2% pectinase in Murashige and Skoog (MS) medium. The protoplasts were washed and incubated in conditioned MS medium supplemented with 0.1 M mannitol and 0.01 M sucrose in microcultures. The amount of cell wall material deposited on the surface of the living single protoplast was measured with the polarizing microscope and photovolt meter. The refracted light was measured as arbitrary units of photovolts every 12 h. The cell wall regeneration was detected after 24 h of incubation. Maximum deposition of cell wall cellulose material was achieved after 60 h and measured 18.4 photovolt units for cells with diameter of 20 to 40 microns (μ) and 22 photovolt units for cells with diameter of 41 to 60 μ. Mitotic cell divisions started in 12 out of 174 reconstituted cells at the 4th or 5th day of incubation. After nuclear division, new cell wall...

Vegetative propagation of Freesia through the isolation of shoots in vitro

Abstract: Shoots from the cvs Ballerina, Aurora, Rijnveld's Golden Yellow and Rose Marie, originating from excised flower-buds, were grown in vitro and were induced to form shoots on a modified Murashige and Skoog medium containing PBA and IAA. The greatest number of new shoots/excised shoot was obtained when the medium contained PBA at 5 mg/l and IAA at 0.1 mg/l. The number of shoots formed depended on the cv. tested. Subcultured shoots, grown on a medium with IAA but without PBA, could be rooted easily and viable plants were obtained. (Abstract retrieved from CAB Abstracts by CABI’s permission)

In Vitro Culture of Young Embryo in Polyembryonic Citrus (I)

Abstract: Polyembryonic satsuma mandarin (Citrus unshiu Marc.) was crosspollinated with trifoliate orange (Poncirus trifoliata L.).Embryos were isolated from immature seeds 90, 120, and 140 days after pollination.Isolated embryos were divided into the following five stages:Stage I: Globular-shaped embryo (less than 0.1mm in dia.)Stage II: Early heart-shaped embryo (0.1mm-0.3mm in dia.)Stage III: Middle _??_ (0.3mm-0.4mm _??_)Stage IV: Late _??_ (0.4mm-0.5mm _??_)Stage V: Torpedo-shaped embryo (greater than 0.5mm in dia.)These embryos were cultured on Murashige and Skoog (MS) medium and MS medium supplemented with 20% non-autoclaved cucumber juice (C. J.) under aseptic conditions.The growth of embryos and the number of differentiated cotyledons were greater when 90 and 120 day embryos except Stage V were cultured on the medium with cucumber juice whereas no such effects were observed on 140 day embryos.On the contrary, root differentiation and rooting were reduced by the addition of cucumber juice.These findings indicate that there would be two factors or complexes present in cucumber juice; (1) a factor causing both growth and differentiation of cotyledon, (2) a factor causing inhibition of root growth.For the successful rearing of heart-shaped embryos isolated 90 and 120 days after pollination, it appears that the following series of subcultures at one month intervals would be recommended:(1) Initial culture of heart-shaped embryos on MS medium with 20% C. J. and 3% sucrose.(2) Subculture of those embryos on MS medium with 3% sucrose without C. J.(3) Subculture of them on MS medium with 1.5% sucrose.(4) Transplanting young seedlings to vermiculite.Zygotic seedlings with trifoliate leaves obtained were about 40 to 60% of total seeds examined and such seedlings came only from embroys in Stage IV and V.