Showing papers on "Murashige and Skoog medium published in 1979"

Differentiation in Lilium Bulbscales Grown in vitro. Effect of Various Cultural Conditions

Abstract: Tissue cultures of Lilium auratum Lindl and L speciosum Thunb, which were derived from bulbscales, all appeared to differentiate organs The effect of cultural conditions on the differentiation of bulblets and roots was examined The best material for bulblet formation was bulbscales of intact or in vitro produced bulblets The optimum temperature was 20°C and optimum pH was 6 Effect of irradiance on organ formation was not obvious but leaf emergence was stimulated Higher kinetin concentrations stimulate the formation of numerous bulbscalcs High NAA concentrations induce roots On the other hand kinetin inhibits the NAA effect on root formation A high sucrose concentration stimulated organ formation, but the number of bulblets was at a constant level in the medium containing between 10 and 90 g/l of sucrose The formation of bulblets and their growth were stimulated at increasing strength of Murashige-Skoog's (MS) medium, but the length of roots was inhibited Inter action of strength of MS medium and sucrose concentration was examined High concentration of both components stimulated bulb lei growth, but the second strength of MS medium containing 90 or 120 g/l sucrose stimulated callus induction and inhibited the growth of bulblets Maximum growth took 100 days for bulblets and about 50 days for roots The change of fresh weight/dry weight ratio during differentiation is also discussed

In vitro culture of Cinchona ledgeriana L.

Abstract: SummaryIn vitro cultures of Cinchona ledgeriana were established from seedling stock grown on a modified Murashige and Skoog medium. The addition of phloroglucinol to the medium promoted growth and, within the range 40·5 to 181·5 mg 1-1, there was no permanent effect of concentration on the growth promotion.

Differentiation in Callus Cultures of Leaf of Two Species of Trigonella

Abstract: Callusing of leaf segment of Trigonella corniculata L. and Trigonelta foenum graecum L. was observed on Murashige and Skoog's (MS) medium and White's (W) medium supplemented with naphthalene acetic acid (NAA; 0.5 mg/1) and coconut milk (15% v/v). Root, shoot and development of isolated leaf occurred in the calli of both the species on MS medium containing NAA amd coconut milk. Omission of coconut milk with subsequent addition of casein hydrolysale (CH) increased the number of differentiated organs per callus. In both the materials higher differentiation rate was observed at low irradiance than at high irradiance or in complete darkness. Initially the cells were diploid but at the 5th passage the calli became mixoploid wilh predominantly diploid cell populations. The differentiated organs were diploid.

Experimental induction of triploid plants ofPhysalis through anther culture

Abstract: Anther culture ofPhysalis minima L. (2 n=48) was successful and embryos and plantlets could be obtained 5–6 weeks after culture. MS medium containing CM was essential for pollen division. 16.7% of the cultured anthers produced plantlets. All the pollen plantlets were triploid (3 n=72). Regeneration of multiple shoot buds was obtained from cultured leaf and stem expiants of pollen plantlets.

The growth of sugarcane downy mildew fungus in tissue culture

Abstract: Dual culture of sugarcane downy mildew fungus (Sclerospora sacchari Miyake) was established by placing a diseased sugarcane explant (shoot apex or spindle leaf) on a modified Murashige and Skoog medium containing 3 ppm 2,4-dichlorophenoxyacetic acid. After 3 weeks incubation, dense mycelia grew both on and in the callus which had developed. The use of different varieties or illumination periods did not affect the growth of the fungus. The hyphae ceased to grow when the infected callus was subcultured. A workable procedure was devised which enabled the hyphae to proliferate on a healthy tissue when diseased tissue was placed near it. Explants excised from either resistant or susceptible varieties could nurse the growth of the fungus, indicating that resistant genes may not be expressed in tissue culture. Histological studies revealed that the callus originated from mesophyll cells in leaf and parenchymatous cells of shoot apical tissue. Intercellular and intracellular hyphae were more concentrated in the p...

Induction of root and shoot formation from root meristems ofPetunia hybrida

Abstract: A procedure has been developed for the induction of root or shoot formation from root meristems of germinated seeds ofPetunia hybrida. Root formation was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 6-benzylaminopurine (BA) (0–0.5 mg/l) and naphtaleneacetic acid (NAA) (0.05–2.0 mg/l). Induction of predominantly shoot formation was obtained on MS medium containing the following combinations of hormones (in mg/l): 0.05–0.5 NAA and 0.25–2.0 BA. Complete plant formation was obtained after rooting of the shoots on MS medium supplemented with IAA (0–2.0 mg/l) or NAA (0-0.5 mg/l).

Influence of Cytokinins and Temperature on Development of Asparagus plumosus Shoot Tips in vitro

Abstract: Lateral shoot tips from young shoots of Asparagus setaceus (Kunth) Jessop (syn. A. plumosus Baker) were grown on a modified Murashige and Skoog medium. PBA was the most effective cytokinin for growth and development of Asparagus plumosus shoot tips. The optimal range of PBA was between 0.02 and 2 mg/1. Zeatin was less effective, and kinetin, BA and 2iP were poor as cytokinins for A. plumosus. The optimal temperature for growth and development was 21°C. There was interaction between the temperature and the PBA concentration. With increasing temperature from 17 to 24°C the need for PBA increased from 0.2 to 2 mg/1.