Showing papers on "Murashige and Skoog medium published in 1983"
TL;DR: A morphogenetically competent suspension culture was derived from embryonic axes of Glycine max cv.
Abstract: A morphogenetically competent suspension culture was derived from embryonic axes of Glycine max cv. Mitchell. The cultural history included visual selection for nonfriable, embryo-like structures, recurrent selection in a regime of 2,4-dichlorophenoxyacetic acid exposure and withdrawal, and the replacement of the nitrogen in a Murashige and Skoog salts-based medium with 20 millimolar ammonium citrate. The embryoids produced by this suspension are capable of completing plantlet development. The suspension can be maintained by serial subculture.
145 citations
TL;DR: Calli grown on MSC3 medium, when transferred to half-MS medium containing 15 g 1−1 sucrose, produced tiny plantlets without forming coleoptiles, suggesting precocious germination of somatic embryos, and the regenerates included morphological variants.
Abstract: Plants, regenerated from callus cultures of sugarcane (Saccharum officinarum L) clone IJ76-316, originated through somatic embryogenesis Callus cultures were established from primordial leaves and apical meristems on Murashige and Skoog medium (MS) supplemented with 3 mg 1−1 2,4-dichlorophenoxy acetic acid and 100 ml 1−1 coconut water (MSC3) Nodular calli formed within 2 weeks of culture Calli were maintained on MSC3 medium by transfer every 3 to 4 weeks Somatic embryogenesis occurred after 10 weeks culture of callus on MSC3 medium Somatic embryogenesis was also observed in cell suspension cultures initiated from calli maintained on MSC3 and then cultured in half strength MS liquid medium supplemented with 05 mg 1−1 2,4-D Somatic embryos produced coleoptiles and shoots 2 to 4 weeks after transfer to MS medium supplemented with 100 ml 1−1 coconut water (MSC), and produced complete plantlets within 4 weeks of further culture on half-strengh MS medium (half-MS) with 30 g 1−1 sucrose Calli grown on MSC3 medium, when transferred to half-MS medium containing 15 g 1−1 sucrose, produced tiny plantlets, circa 4–10 mm, without forming coleoptiles, suggesting precocious germination of somatic embryos The regenerates included morphological variants
88 citations
TL;DR: Multiple shoots were formed from seedling cultures of Alnus glutinosa (L.) Gaertn on modified Murashige and Skoog medium supplemented with 0.5–5 μM of benzyladenine (BAP).
58 citations
TL;DR: The production of callus from leaf tissue of Triticum aestivum and regeneration of whole plants from this callus are reported and Root development was achieved by transfer of these cultures onto MS medium containing half-salt concentration and no added growth regulators.
55 citations
TL;DR: It was found that an increased amount of KNO3 did not raise the rate of embryoid formation, and embryoids most likely originated from single cells which, however, may be part of a larger proembryonal cell complex.
Abstract: The development of embryoids on punched leaf expiants fromCoffea canephora was studied. Both culture conditions as well as structural changes were investigated. The expiants were placed on a modified Murashige and Skoog medium with different concentrations of KNO3. It was found that an increased amount of KNO3 did not raise the rate of embryoid formation.
54 citations
TL;DR: Hypocotyl explants of cucumber produced callus when grown in Murashige and Skoog medium with 0.5 or 1.0 μM benzyladenine and 2,4-D, and flowers that were formed in vitro were either staminate or pistillate.
51 citations
TL;DR: The hormonal control of growth and development of meristem tips, as well as the significance of in vitro propagation for long-term storage and clonal selection of grapevine cultivars, are discussed.
46 citations
TL;DR: Embryoids developed into plantlets when subcultured on a half-strength Murashige and Skoog medium supplemented with indoleacetic acid (IAA) and casein hydrolysate and callus derived from immature pericarp tissue or peduncles did not show morphogenetic responses.
39 citations
TL;DR: Proliferating shoot cultures were established from shoot tips and nodal bud segments excised from seedlings germinated aseptically and cultured on Murashige and Skoog medium supplemented with BAP plus NAA.
Abstract: SummaryProliferating shoot cultures were established from shoot tips and nodal bud segments excised from seedlings germinated aseptically and cultured on Murashige and Skoog medium supplemented with BAP plus NAA. Shoot tip necrosis occurred in some cultures. Cultured shoots were rooted in vitro using MS medium (half strength macronutrients) containing IBA for root initiation, followed by subculture onto hormone-free medium for root development. Rooted shoots were readily established in peat-based compost.
38 citations
TL;DR: Callus tissue cultures of Medicago sativa L. were initiated on Murashige and Skoog medium supplemented with 2 mg·l -1 2,4-D from seedling cotyledons and hypocotyls and the possibility to propagate a regenerating culture of Med Chicago sativa as a continuous source of plantlets are discussed.
36 citations
TL;DR: Callus cultures of two cultivars of Vigna aconitifolia were raised and their growth and differentiation studied, finding best differentiation of shoot buds was observed on a medium with K (23.2 μM) and IAA (5.7 μM).
Abstract: Callus cultures of two cultivars of Vigna aconitifolia (IPCMO-926, RDM-120) were raised and their growth and differentiation studied. In IPCMO-926 callus cultures, numerous shoot buds differentiated on MS medium with BA (0.4–22.2 μM) alone or in combination with IAA (5.7 μM). In RDM-120 best differentiation of shoot buds was observed on a medium with K (23.2 μM) and IAA (5.7 μM). Kinetin alone, however, induced rhizogenesis in callus cultures. In suspension cultures of IPCMO-926 embryoids differentiated on MS medium with K (0.5 μM) and 2,4-D (0.4 and 0.9 μM).
TL;DR: Evaluation of a range of enzyme formulations showed that almost all cells could be converted to viable protoplasts in 9 hours with the combination of 0.5% each of Onozuka R-10, Macerozyme R- 10, Hemicellulase, Driselase, and bovine serum albumin in osmoticum.
Abstract: Protoplasts were prepared from exponentially growing cell suspension cultures derived from Pinus taeda seedlings. The cell suspension was derived from callus that had been initiated on solid Murashige and Skoog medium supplemented with 2mg/1 2,4-D, and maintained in liquid LM medium (D.C. Verma et al, Proceedings of the 5th International Congress of Plant Tissue and Cell Culture, Tokyo, Japan, 1982, p. 59–60). Evaluation of a range of enzyme formulations showed that almost all cells could be converted to viable protoplasts in 9 hours with the combination of 0.5% each of Onozuka R-10, Macerozyme R-10, Hemicellulase, Driselase, and bovine serum albumin in osmoticum (0.4 M mannitol, 0.2 M sorbitol, 1.5 mM CaCl2, 0.7 mM KCl). Protoplast purification required only simple washing which was achieved without damaging centrifugation. When cultured in LM medium supplemented with 1.5mM CaCl2, 10 mM arginine, 10mM glutamine and 5 mM asparagine, cell wall formation was observed after 48 hours. The cultured protoplasts produced numerous colonies in 3 weeks which continued to grow in liquid medium.
TL;DR: Maize (Zea mays L.) A188 calluses derived from leaf tissues of in vitro grown seedlings produced green leafy structures and subsequently plantlets upon transfer to N6 medium containing 2mg/1 2,4-D and 0.1 mg/1 zeatin.
Abstract: Maize (Zea mays L.) A188 calluses derived from leaf tissues of in vitro grown seedlings were initiated and maintained on Murashige and Skoog medium supplemented with 2 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D). The calluses produced green leafy structures and subsequently plantlets upon transfer to N6 medium containing 2 mg/1 2,4-D and 0.1 mg/1 zeatin. An embryo-like structure with a green prominent coleoptile and a scutellum-like body was also obtained.
TL;DR: Callus culture and plant regeneration through somatic embryogenesis have been obtained in Coronilla varia using media supplemented with 2,4-D and subculture on MS containing 1 mg/l 2-iP and 0.1mg/l IAA.
Abstract: Callus culture and plant regeneration through somatic embryogenesis have been obtained in Coronilla varia. Media used were UM (25) supplemented with 2 mg/l 2,4-D followed by subculture on MS (18) containing 1 mg/l 2-iP and 0.1 mg/l IAA. Embryoids developed into complete plantlets on filter paper saturated with hormone-free MS medium.
TL;DR: The authors obtained calluses and dihaploid plantlets from the anthers of ten autotetraploid genotypes when they contained pollen at the uninucleate stage by culturing them on Murashige and Skoog medium supplemented with different hormones and added sucrose.
Abstract: Calluses and dihaploid plantlets were obtained from the anthers of ten autotetraploid genotypes when they contained pollen at the uninucleate stage by culturing them on a Murashige and Skoog medium supplemented with different hormones and added sucrose. The anther response depended more on the genotype than on the composition of the media.
TL;DR: Protoplast-derived colonies formed callus which readily regenerated shoots on fransfer to Murashige and Skoog based agar medium, and Leaf explants also showed a marked capacity for shoot regeneration in culture.
Abstract: Mesophyll protoplasts obtained from leaves of shoot cultures of Rehmannia glutinosa were cultured in Murashige and Skoog (1962) liquid or liquid-over-agar medium containing 2.0 mg L−1 naphthaleneacetic acid and 0.5 mg L−1 benzylamino purine. An amino acid mixture of glutamine, arginine, glycine, and aspartic acid promoted sustained protoplast division, with an average plating efficiency of 27%. Protoplast-derived colonies formed callus which readily regenerated shoots on fransfer to Murashige and Skoog based agar medium with 2.0 mg L−1 indoleacetic acid and 1.0 mg L−1 benzylamino purine. Leaf explants also showed a marked capacity for shoot regeneration in culture.
TL;DR: Calli originated from different B. ternifolia tissues do not have the same biochemical dedifferentiated state, and when the calli were supplied with glutamine as the sole nitrogen source, the activity of glutamate synthase increased in leaf callus but was almost completely inhibited in root callus.
Abstract: Calli derived from leaves and radicles of B. ternifolia were grown on Murashige and Skoog (MS) basal medium, and the effects of different nitrogen sources on the rate of callus growth and on the enzymes related to nitrogen assimilation were studied. Ammonium alone did not support callus growth unless a Krebs-cycle intermediate was added to the medium. The activities of glutamine synthetase (EC 6.3.1.2), glutamate synthase (EC 1.4.7.1), and glutamate dehydrogenase (EC 1.4.1.2) were measured in homogenates of callus grown on media supplied with different nitrogen sources. The results indicate that leaf and root calli have similar levels of these enzymes when grown on MS medium (Murashige and Skoog 1962. Physiol. Plant. 15, 473–497). However, when the calli were supplied with glutamine as the sole nitrogen source, the activity of glutamate synthase increased in leaf callus but was almost completely inhibited in root callus. The results indicate that calli originated from different B. ternifolia tissues do not have the same biochemical dedifferentiated state.
TL;DR: Root cultures from several species of the Solanaceae were initiated and subcultured on Murashige and Skoog medium without growth regulators and the effect of several concentrations of auxin and cytokinins on the number of shoots generated by two highly responsive species is described.
Abstract: Root cultures from several species of the Solanaceae were initiated and subcultured on Murashige and Skoog medium without growth regulators. Direct shoot regeneration was observed in four different species. The effect of several concentrations of auxin (IAA) and cytokinins (BAP, zeatin) on the number of shoots generated by two highly responsive species (Nicotiana exigua, N. debneyi) is described.
TL;DR: The experiments aimed at efficiently regenerating plants from cell suspension-derived protoplasts of S. melongena could be a useful species for somatic hybridization.
Abstract: Several members of the genus Solanum have been successfully used in somatic hybridization studies (1, 2, 3). We have established callus and cell suspension cultures of Solanum melongena and some wild Solanum species. High frequencies of somatic embryogenes is were obtained in S. melongena cell suspension cultures grown in MS medium in the presence of 10 mg/L NAA (4). Plants could be regenerated from these embryos suggesting that S. melongena could be a useful species for somatic hybridization. In this presentation, we describe our experiments aimed at efficiently regenerating plants from cell suspension-derived protoplasts of this species.
TL;DR: In this article, the influence of light, temp, growth regulators, and sugar and macrosalt concn on the growth and morphogenesis of leaf and axis explants of young Shorea curtisii, S. obtusa and Dipterocarpus grandiflorus was made.
Abstract: A study was made of the influence of light, temp., growth regulators, and sugar and macrosalt concn. on the growth and morphogenesis of leaf and axis explants of young Shorea curtisii, S. obtusa and Dipterocarpus grandiflorus. Terminal and axillary buds grew best on half strength Murashige and Skoog medium. Leaf explants formed more callus on full strength medium, when containing part of the midrib and when taken from the lower half of the leaf. More than 95% of D. grandiflorus explants were infected by a fungus apparently present in the parent plant. (Abstract retrieved from CAB Abstracts by CABI’s permission)
TL;DR: It is suggested that the effectiveness of medium for protoplast culture depends on endogenous conditions of the plant before the isolation of protoplasts, and that calluses with a shorter period of culture were suitable for achieving a higher rate of normal shoot formation with abundant shoots per callus.
Abstract: The purpose of this study was to enhance the reproducibility of plating efficiency in leaf protoplasts of Lycopersicon peruvianum and determine the optimum concentration of plant hormones for shoot formation from calluses derived from protoplasts. Cell division was constantly induced in leaf protoplasts isolated from the plants grown at a low temperature (20°C) and under an illumination of 2, 500lux (16hr day length), in addition to high humidity conditions and moderate supply of nutrients. Plating efficiency was largely influenced by the leaf age, type of plant hormones, composition of medium and plants for protoplast culture. It is suggested that the effectiveness of medium for protoplast culture depends on endogenous conditions of the plant before the isolation of protoplasts. The optimum concentration of indoleacetic acid (IAA) and benzyladenine (BA) in MS medium (MURASHIGE and SKOOG, 1962) for shoot formation from calluses derived from protoplasts was found to be about 2.0 mg/l BA with or without the addition of a small amount of IAA. Calluses with a shorter period of culture were suitable for achieving a higher rate of normal shoot formation with abundant shoots per callus.
TL;DR: Mesophyll protoplasts isolated from leaves of axenic shoot cultures of Nicotiana bonariensis underwent sustained cell division and concomitant novel cellular organizational patterns when cultured in liquid Murashige and Skoog medium and the potential value of somatic variants are discussed.
TL;DR: Rapid development of axillary buds from shoot-tips and nodes of 18 cultivars of Fuchsia hybrida was obtained on solid Murashige and Skoog medium with BAP and an auxin, and NAA as the auxin appeared to be more active than IAA or IBA.
TL;DR: Tissue treated with 1% CO2 consistently produced more shoots than the ambient controls when placed on Murashige and Skoog medium containing 3mg/l indole-3-acetic acid and 0.3 mg/l kinetin, indicating CO2-enhancement of shoot formation.