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Mycelium
About: Mycelium is a research topic. Over the lifetime, 8923 publications have been published within this topic receiving 170993 citations.
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TL;DR: The lipid and fatty acid compositions of Glomus intraradices and G. claroideum mycelia, extracted from quartz sand in a compartmentalized growth system, were analysed and it was found that the fungal biomass was equally distributed between the externalMycelium and the internal mycelium in the host root.
158 citations
TL;DR: Analysis of 26-day-old mycelia showed that the neutral sugars galactose, glucose, mannose, and galactosamine were predominant, and none of the polysaccharides exhibited cytotoxic effects.
Abstract: Polysaccharides were extracted from fruiting bodies and cultured mycelia from five Antrodia camphorata strains Polysaccharide profiles of the five strains, as determined by high-performance anion-exchange chromatography, showed varying yields and composition of neutral sugars A camphorata fruiting bodies also had different polysaccharide patterns compared to the cultured mycelium Analysis of 26-day-old mycelia showed that the neutral sugars galactose, glucose, mannose, and galactosamine were predominant All mycelia polysaccharide preparations exhibited anti-hepatitis B virus activity Polysaccharides from strain B86 at a concentration of 50 μg ml−1 showed the highest level of anti-hepatitis B surface antigen effect, which was higher than α-interferon at a dosage of 1000 U ml−1 Only strains B86 and 35398 had substantial anti-hepatitis B e antigen activities None of the polysaccharides exhibited cytotoxic effects
158 citations
TL;DR: In the long term, the positive effect of fire on bacteria was nullified except on the sporulating ones; fungal propagules, but not mycelium, reached the unburned soil values, cyanobacteria and algae also increased and soil incubation both improved the beneficial and diminished the negative fire effect on the microbiota.
157 citations
TL;DR: C. minitans will survive and spread in soil for at least 2 yr and continue to give some control of disease but details of its ecology are still uncertain, and the reasons for the lack of commercial interest in this biological control agent are discussed.
156 citations
TL;DR: Image analysis of morphological development of A. niger during the cultivation process showed that the microparticles influence the morphology by collision‐induced disruption of conidia aggregates and probably also the hindrance of new spore–spore interactions in the very early stage of the process.
Abstract: Supplementation with silicate microparticles was used as novel approach to control the morphological development of Aspergillus niger, important as the major world source of citric acid and higher-value enzymes, in submerged culture. With careful variation of size and concentration of the micromaterial added, a number of distinct morphological forms including pellets of different size, free dispersed mycelium, and short hyphae fragments could be reproducibly created. Aluminum oxide particles similarly affected morphology, showing that this effect is largely independent of the chemical particle composition. Image analysis of morphological development of A. niger during the cultivation process showed that the microparticles influence the morphology by collision-induced disruption of conidia aggregates and probably also the hindrance of new spore-spore interactions in the very early stage of the process. Exemplified for different recombinant A. niger strains enzyme production could be strongly enhanced by the addition of microparticles. Linked to the formation of freely dispersed mycelium, titers for glucoamylase (GA) expressed as intracellular enzyme (88 U/mL) and fructofuranosidase secreted into the supernatant (77 U/mL), were up to fourfold higher in shake flasks. Moreover, accumulation of the undesired by-product oxalate was suppressed by up to 90%. The microparticle strategy could be successfully transferred to fructofuranosidase production in bioreactor, where a final titer of 160 U/mL could be reached. Using co-expression of GA with green fluorescent protein, enzyme production was localized in the cellular aggregates of A. niger. For pelleted growth, protein production was maximal only within a thin layer at the pellet surface and markedly decreased in the pellet interior, whereas the interaction with the microparticles created a highly active biocatalyst with the dominant fraction of cells contributing to production.
156 citations