Topic
Mycelium
About: Mycelium is a research topic. Over the lifetime, 8923 publications have been published within this topic receiving 170993 citations.
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TL;DR: A microscopic and biochemical comparison was made of the distribution, quantity and composition of lipid in roots of onion, clover and ryegrass infected with the VA mycorrhizal fungus, Glomus mosseae, in uninfected roots and in the external mycelium from infected, aseptically grown onion roots.
Abstract: SUMMARY
A microscopic and biochemical comparison was made of the distribution, quantity and composition of lipid in roots of onion, clover and ryegrass infected with the VA mycorrhizal fungus, Glomus mosseae, in uninfected roots and in the external mycelium from infected, aseptically grown onion roots. Abundant oil-droplets were observed in the internal and external fungal mycelium, in the vesicles and chlamydospores, and adjacent to the appressoria at entry points. Mycorrhizal roots contained significantly more total lipid than uninfected roots. In estimations of separated lipid classes, the mycorrhizal roots consistently showed higher levels of triglyceride than non-inycorrhizal roots. External mycelium contained high levels of neutral lipids, especially triglyceride, diglyceride and free fatty acids. Compared with uninfected roots, mycorrhizal onion roots contained higher levels of diphosphatidyl glycerol, phosphatidyl serine, phosphatidyl ethanolamine, phosphatidy) choline, phosphatidyl glycerol, phosphatidic acid and phosphatidyl inositol. With the exception of phosphatidyl inositol and phosphatidic acid, these were all prominent components of G. mosseae mycelium.
100 citations
TL;DR: Real-time RT-PCR was used to quantify the expression of 18 transcripts originating from germinating cysts, relative to the constitutively expressed actB gene, in vegetative mycelium, germinated cysts and at three time-points post-inoculation of potato cultivar Bintje.
100 citations
TL;DR: Strain PY-1 produces antifungal substances that suppress the mycelial growth of Sclerotinia sclerotiorum and many other plant pathogenic fungi tested; the highest antagonistic activity was detected at 72 h when cultured in a 250-ml flask containing 80 ml potato dextrose broth.
Abstract: This paper reports the isolation from soil of Penicillium strain PY-1 with strong antagonistic activity against plant pathogenic fungi. On the basis of its morphological characteristics and the sequence of the ITS region, strain PY-1 was identified as P. oxalicum. Strain PY-1 produces antifungal substances that suppress the mycelial growth of Sclerotinia sclerotiorum and many other plant pathogenic fungi tested; the highest antagonistic activity was detected at 72 h when cultured in a 250-ml flask containing 80 ml potato dextrose broth. Compared with carbendazim, the relative activity of the antifungal substances produced by strain PY-1 was approximately 4 μg active ingredient (a.i.) per milliliter. The antifungal substances were extracted with ethyl acetate and further separated by high-performance liquid chromatography (HPLC); at least two active components were discovered. The ability to control plant disease with strain PY-1 was confirmed with S. sclerotiorum, a widespread pathogenic fungus that attacks rapeseed (Brassica napus) and other plants. Spores (106 or 107 ml−1) and filtrate (tenfold diluted or undiluted) of strain PY-1 could significantly suppress infection and/or the extent of infection by S. sclerotiorum of plants at seven-true-leaves stage. The potential of strain PY-1 for identifying new antibiotics to control fungal disease and for biological control of plant disease, for example oilseed rape stem rot, is discussed.
100 citations
TL;DR: It is concluded that carbon flow in the AM symbiosis is normally unidirectional from plant to fungus and that while carbon is translocated by the fungus from one metabolically active root system to another, it remains within the intraradical mycelium (IRM).
Abstract: Summary • Carbon transfer from fungus to plant in the arbuscular mycorrhizal (AM) symbiosis has been reported, but its significance and even its existence have been called into question and the issue remains controversial. We investigated carbon movement from fungus to plant and from one mycorrhizal root system to another via a common AM fungal network in monoxenic cultures to avoid limitations of some previous studies. • 13 C and 14 C labeled substrates were supplied to functioning in vitro AM mycorrhizas between Ri T-DNA transformed carrot ( Daucus carota ) roots and Glomus intraradices to follow carbon movement into and between host and fungal metabolite pools. • Fungal triacylglycerol and trehalose were labeled when permeant substrates were supplied to the extraradical mycelium (ERM), but host-specific compounds in the roots did not become labeled. When labeled glucose was provided to a donor root system, label moved to recipient roots via a common AM fungal network but remained in fungal compounds. •W e conclude that carbon flow in the AM symbiosis is normally unidirectional from plant to fungus and that while carbon is translocated by the fungus from one metabolically active root system to another, it remains within the intraradical mycelium (IRM).
100 citations
TL;DR: In vitro trials were conducted to evaluate the effect of several organic and inorganic salt compounds and two commercial fungicides on mycelial growth, sporulation and spore germination of Alternaria alternata, Botrytis cinerea, Fusarium solani var.
100 citations