Showing papers on "Nanobiotechnology published in 2002"

Self‐assembling Protein Systems: Microbial S‐layers

Abstract: Introduction Historical Outline Occurrence and Ultrastructure Isolation and Chemical Characterization Molecular Biology, Genetics and Biosynthesis Assembly and Morphogenesis Self-assembly in vivo Self-assembly in vitro Functional Aspects Biodegradation Production of S-layer Proteins Application of S-layer Proteins S-layer Ultrafiltration Membranes S-layers as Matrix for the Immobilization of Functional Macromolecules S-layer-based Dipsticks Supramolecular Structures Generated by Oriented Recrystallization of S-layer Fusion Proteins on Supports Precoated with SCWP S-layers as Templates for the Formation of Regularly Arranged Nanoparticles S-layers as Supporting Structures for Functional Lipid Membranes (Planar Membranes and Liposomes) S-layers for Vaccine Development Outlook and Perspectives Patents Keywords: affinity microparticles; artificial virus; biomimetic membrane; biomimetics; biosensor; glycan biosynthesis; glycan structure; immobilization; nanoarrays; nanobiotechnology; nanotechnology; patterning elements; protein lattices; secondary cell; wall polymers; self-assembly; S-layer fusion proteins; S-layer glycoprotein; solid phase immunoassays; supported lipid membrane; supramolecular structures; surface layer; ultrafiltration

A novel nonviral nanoparticle gene vector: Poly-L-lysine-silica nanoparticles

Abstract: DNA delivery is a core technology for gene structure and function research as well as clinical settings. The ability to safely and efficiently targeted transfer foreign DNA into cells is a fundamental goal in biotechnology. With the development of nanobiotechnology, nanoparticle gene vectors brought about new hope to reach the goal. In our research, silica nanoparticles (SiNP) were synthesized first in a microemulsion system polyoxyethylene nonylphenyl ether (OP-10)/cyclohexane/ammonium hydroxide, at the same time the effects of SiNP size and its distribution were elucidated by orthogonal analysis; then poly-L-lysine (PLL) was linked on the surface of SiNP by nanoparticle surface energy and electrostatically binding; lastly a novel complex nanomateial—poly-L-lysine-silica nanoparticles (PLL-SiNP) was prepared. The analysis of plasmid DNA binding and DNase I enzymatic degradation discovered that PLL-SiNP could bind DNA, and protect it against enzymatic degradation. Cell transfection showed that PLL-SiNP could efficiently transfer PEGFPC-2 plasmid DNA into HNE1 cell line. These results indicated that PLL-SiNP was a novel nonviral nanoparticle gene vector, and would probably play an important role in gene structure and function research as well as gene therapy.