Topic
Nanobiotechnology
About: Nanobiotechnology is a research topic. Over the lifetime, 796 publications have been published within this topic receiving 46309 citations. The topic is also known as: bionanotechnology & nanobiology.
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TL;DR: This review covers the emerging field of nanobiotechnology, in which nanoparticles are applied to the analysis of biomolecules, and discusses the next generation of nanoparticles that will be utilized in the life sciences, such as nanodots and carbon nanotubes.
507 citations
TL;DR: It is shown that chemical reactions with single molecules can be performed and imaged at a local position on a DNA origami scaffold by atomic force microscopy and demonstrate the feasibility of post-assembly chemical modification of DNA nanostructures and their potential use as locally addressable solid supports.
Abstract: DNA nanotechnology and particularly DNA origami, in which long, single-stranded DNA molecules are folded into predetermined shapes, can be used to form complex self-assembled nanostructures. Although DNA itself has limited chemical, optical or electronic functionality, DNA nanostructures can serve as templates for building materials with new functional properties. Relatively large nanocomponents such as nanoparticles and biomolecules can also be integrated into DNA nanostructures and imaged. Here, we show that chemical reactions with single molecules can be performed and imaged at a local position on a DNA origami scaffold by atomic force microscopy. The high yields and chemoselectivities of successive cleavage and bond-forming reactions observed in these experiments demonstrate the feasibility of post-assembly chemical modification of DNA nanostructures and their potential use as locally addressable solid supports.
488 citations
TL;DR: A novel method using a pH 3.0 citrate buffer to complete the attachment of thiolated DNA to gold nanoparticles and reveals the fundamental kinetics of AuNP aggregation versus DNA adsorption, providing a novel means to modulate the interactions between DNA and AuNPs.
Abstract: The attachment of thiolated DNA to gold nanoparticles (AuNPs) has enabled many landmark works in nanobiotechnology. This conjugate chemistry is typically performed using a salt-aging protocol where, in the presence of an excess amount of DNA, NaCl is gradually added to increase DNA loading over 1–2 days. To functionalize large AuNPs, surfactants need to be used, which may generate difficulties for downstream biological applications. We report herein a novel method using a pH 3.0 citrate buffer to complete the attachment process in a few minutes. More importantly, it allows for quantitative DNA adsorption, eliminating the need to quantify the number of adsorbed DNA and allowing the adsorption of multiple DNAs with different sequences at predetermined ratios. The method has been tested for various DNAs over a wide range of AuNP sizes. Our work suggests a synergistic effect between pH and salt in DNA attachment and reveals the fundamental kinetics of AuNP aggregation versus DNA adsorption, providing a novel ...
460 citations
TL;DR: Uniform core/shell nanoparticles, consisting of a silica layer coating and pigments or magnetite core, using a water‐in‐oil microemulsion method, are developed, highly luminescent and photostable with the size ranging from 5 nm to 400 nm.
Abstract: We have developed uniform core/shell nanoparticles, consisting of a silica layer coating and pigments or magnetite core, using a water-in-oil microemulsion method. The nanoparticles are highly luminescent and photostable with the size ranging from 5 nm to 400 nm. Bioconjugation of these silica nanoparticles adds unique biofunctions with various molecules such as enzymes, antibodies, and DNA molecules. Significant advantages have been shown in using bioconjugated nanoparticles for biosensing and bioimaging, such as cell staining, DNA detection and separation, rapid single bacterium detection, and biotechnological application in DNA protection.
442 citations
TL;DR: The application of molecular self‐assemblies of synthetic peptides as nanometer‐scale building blocks in devices is robust, practical, and affordable due to their advantages of reproducibility, large‐scale production ability, monodispersity, and simpler experimental methods.
Abstract: In nature, biological nanomaterials are synthesized under ambient conditions in a natural microscopic-sized laboratory, such as a cell. Biological molecules, such as peptides and proteins, undergo self-assembly processes in vivo and in vitro, and these monomers are assembled into various nanometer-scale structures at room temperature and atmospheric pressure. The self-assembled peptide nanostructures can be further organized to form nanowires, nanotubes, and nanoparticles via their molecular-recognition functions. The application of molecular self-assemblies of synthetic peptides as nanometer-scale building blocks in devices is robust, practical, and affordable due to their advantages of reproducibility, large-scale production ability, monodispersity, and simpler experimental methods. It is also beneficial that smart functionalities can be added at desired positions in peptide nanotubes through well-established chemical and peptide syntheses. These features of peptide-based nanotubes are the driving force for investigating and developing peptide nanotube assemblies for biological and non-biological applications.
426 citations