Showing papers on "Neurosphere published in 1979"
TL;DR: Evidence is presented indicating that the absence of expression of SV40 genetic information in nondifferentiated teratocarcinoma cells reflects an inability to generate stable viral mRNA, which seems to be correlated with a deficiency in the splicing of viral mRNA.
Abstract: UNDIFFERENTIATED murine teratocarcinoma stem cells do not support simian vacuolating virus 40 (SV40) or polyoma virus (Py) replication or even the expression of the early papovavirus proteins. These undifferentiated cells are also entirely refractory to infection with ecotropic murine C-type viruses. If the stem cells are allowed to differentiate to a variety of somatic cell types, they become susceptible to infection by SV40, Py and ecotopic murine C-type viruses1–4. In contrast, adenovirus type 2 can infect and replicate in the undifferentiated stem cells, although not as efficiently as it can in the differentiated mouse cells5 and certainly much less efficiently than it does in human cells. Studies on the mechanism of the resistance of the undifferentiated teratocarcinoma cells to infection by SV40 has shown that the block is not at the level of virus adsorption, penetration uncoating or transport to the nucleus6. We present here evidence indicating that the absence of expression of SV40 genetic information in nondifferentiated teratocarcinoma cells reflects an inability to generate stable viral mRNA. More specifically, the refractoriness of these cells to SV40 seems to be correlated, at least in part, with a deficiency in the splicing of viral mRNA.
81 citations
TL;DR: This chapter presents an overview of neural cell marker, and suggests ways of purifying normal neural cells that can be maintained and studied in culture and new markers that may subdivide the major glial cell types into functional subclasses.
Abstract: Publisher Summary This chapter presents an overview of neural cell marker. To understand the molecular details of how neural cells interact with each other in the developing and mature nervous system, it is necessary to study homogeneous populations of neural cells interacting in vitro . There are two ways of achieving this: one is to study established neural cell lines and the other is to find ways of purifying normal neural cells that can be maintained and studied in culture. Cell surface markers are used not only for cell identification but also for cell separation. In principle, this can be done by either positive or negative selection. Negative selection procedures have been most widely used, and this approach has been used to purify Schwann cells. Having the major neural cell populations specifically marked is not the end of the neural cell marker story. One can anticipate using these and new markers to purify astrocytes, oligodendrocytes, and neurons. New markers may subdivide the major glial cell types into functional subclasses.
18 citations
TL;DR: Multiangle light-scattering measurements in a flow system have been made on stem cells of the mouse testicular teratocarcinoma and on a variety of their differentiated derivatives, but multipotential stem cells cannot be distinguished from stem cells that are restricted in their development to a single pathway.
Abstract: Stem cells of the mouse testicular teratocarcinoma are capable of giving rise in vivo and in vitro to a wide variety of cell and tissue types representative of each embryonic germ layer. Multiangle light-scattering measurements in a flow system have been made on these stem cells and on a variety of their differentiated derivatives. This technique is capable of distinguishing the stem cells from parietal yolk sac cells, visceral yolk sac cells, neuronal cells and squamous cells. However, multipotential stem cells cannot be distinguished from stem cells that are restricted in their development to a single pathway.
7 citations