Topic
Neurosphere
About: Neurosphere is a research topic. Over the lifetime, 5145 publications have been published within this topic receiving 321088 citations.
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TL;DR: The newfound ability to begin to prospectively identify neural stem cells opens an exciting new era for future neural stem cell work, as was true when hemopoetic stem cells were first identified and purified.
122 citations
TL;DR: It is demonstrated that the proliferating cells in the PGZ contribute to neuronal and glial lineages to maintain the structure of the optic tectum in adult zebrafish.
122 citations
TL;DR: GABA's presence as a near-universal signal that may be altered in tumor cells resulting in modified mitotic activity is discussed.
Abstract: Aside from traditional neurotransmission and regulation of secretion, γ-amino butyric acid (GABA) through GABAA receptors negatively regulates proliferation of pluripotent and neural stem cells in ...
122 citations
TL;DR: The results suggest that, under chronic stress conditions, the number of NSCs is regulated by the actions of glucocorticoids and serotonin, and insights into the molecular mechanisms underlying the pharmacological actions of antidepressant drugs are provided.
Abstract: In rodents, adult neurogenesis occurs in the olfactory bulb and the dentate gyrus of the hippocampus. It has been shown that exposure to psychosocial stress reduces cell proliferation in the dentate gyrus. However, little is known about how stress affects the proliferation kinetics of neural stem cells (NSCs) in the subventricular zone (SVZ), which provide new neurons to the olfactory bulb. We utilized a forced-swim model of stress in the mouse and found that chronic stress decreased the number of NSCs in the SVZ. The reduction of NSC number persisted for weeks after the cessation of stress but was reversed by treatment with the antidepressant drugs fluoxetine and imipramine. We demonstrated by in vitro colony-forming neurosphere assay that corticosterone attenuated neurosphere formation by adult NSCs and, in contrast, that serotonin increased the survival of NSCs. In addition, serotonin expanded the size of the NSC pool in the SVZ when it was infused into the lateral ventricle in vivo. These results suggest that, under chronic stress conditions, the number of NSCs is regulated by the actions of glucocorticoids and serotonin. These data provide insights into the molecular mechanisms underlying the pharmacological actions of antidepressant drugs.
122 citations
TL;DR: It is concluded that migration of glioma cells is regulated by topographical cues that affect cell adhesion and gene expression, and cell migration analysis using nanofiber scaffolds could be used to reproduce native mechanisms of migration and to identify antimigratory strategies not disclosed by other in vitro models.
121 citations