Topic
Neurosphere
About: Neurosphere is a research topic. Over the lifetime, 5145 publications have been published within this topic receiving 321088 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: The potential role of nitric oxide activity in the perivascular niche (PVN) using a genetic engineered mouse model of PDGF-induced gliomas and the NO/cGMP/PKG pathway's promotion of stem cell-like character in the tumor PVN may identify therapeutic targets for this subset ofgliomas.
483 citations
TL;DR: It is shown that human spinal motor neurons, but not interneurons, are selectively sensitive to the toxic effect of glial cells carrying an ALS-causing mutation in the SOD1 gene, demonstrating the relevance of these non-cell-autonomous effects to human motor neurons.
482 citations
TL;DR: This work identifies an Olig2-regulated lineage-restricted pathway critical for proliferation of normal and tumorigenic CNS stem cells and shows p21(WAF1/CIP1) is directly repressed by OLIG2 in neural progenitors and gliomas.
482 citations
TL;DR: The embryonic cerebral cortex contains a population of stem-like founder cells capable of generating large, mixed clones of neurons and glia in vitro, and it is reported that the default state of early cortical stem cells is neuronal and that stem cells are heterogeneous in the number of neurons that they generate.
480 citations
TL;DR: It is demonstrated that FGF20, preferentially expressed in the substantia nigra, acts synergistically with FGF2 to increase the number of DA neurons in ES cell-derived neurospheres, and that the transplanted cells functioned as DA neurons and attenuated MPTP-induced neurological symptoms.
Abstract: Parkinson disease (PD) is a neurodegenerative disorder characterized by loss of midbrain dopaminergic (DA) neurons. ES cells are currently the most promising donor cell source for cell-replacement therapy in PD. We previously described a strong neuralizing activity present on the surface of stromal cells, named stromal cell-derived inducing activity (SDIA). In this study, we generated neurospheres composed of neural progenitors from monkey ES cells, which are capable of producing large numbers of DA neurons. We demonstrated that FGF20, preferentially expressed in the substantia nigra, acts synergistically with FGF2 to increase the number of DA neurons in ES cell-derived neurospheres. We also analyzed the effect of transplantation of DA neurons generated from monkey ES cells into 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated (MPTP-treated) monkeys, a primate model for PD. Behavioral studies and functional imaging revealed that the transplanted cells functioned as DA neurons and attenuated MPTP-induced neurological symptoms.
478 citations