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Neutralizing antibody

About: Neutralizing antibody is a(n) research topic. Over the lifetime, 7437 publication(s) have been published within this topic receiving 296219 citation(s). The topic is also known as: NAb & Antibodies, Neutralizing.

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Open accessJournal ArticleDOI: 10.1038/31405
18 Jun 1998-Nature
Abstract: The entry of human immunodeficiency virus (HIV) into cells requires the sequential interaction of the viral exterior envelope glycoprotein, gp120, with the CD4 glycoprotein and a chemokine receptor on the cell surface. These interactions initiate a fusion of the viral and cellular membranes. Although gp120 can elicit virus-neutralizing antibodies, HIV eludes the immune system. We have solved the X-ray crystal structure at 2.5 A resolution of an HIV-1 gp120 core complexed with a two-domain fragment of human CD4 and an antigen-binding fragment of a neutralizing antibody that blocks chemokine-receptor binding. The structure reveals a cavity-laden CD4-gp120 interface, a conserved binding site for the chemokine receptor, evidence for a conformational change upon CD4 binding, the nature of a CD4-induced antibody epitope, and specific mechanisms for immune evasion. Our results provide a framework for understanding the complex biology of HIV entry into cells and should guide efforts to intervene.

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Topics: Envelope glycoprotein GP120 (69%), Chemokine receptor binding (64%), V3 loop (63%) ...read more

2,952 Citations


Open accessJournal ArticleDOI: 10.1128/JVI.68.7.4650-4655.1994
Richard A. Koup1, Jeffrey T. Safrit1, Yunzhen Cao1, C. A. Andrews1  +4 moreInstitutions (1)
Abstract: Virologic and immunologic studies were performed on five patients presenting with primary human immunodeficiency virus type 1 (HIV-1) infection. CD8+ cytotoxic T lymphocyte (CTL) precursors specific for cells expressing antigens of HIV-1 Gag, Pol, and Env were detected at or within 3 weeks of presentation in four of the five patients and were detected in all five patients by 3 to 6 months after presentation. The one patient with an absent initial CTL response had prolonged symptoms, persistent viremia, and low CD4+ T-cell count. Neutralizing antibody activity was absent at the time of presentation in all five patients. These findings suggest that cellular immunity is involved in the initial control of virus replication in primary HIV-1 infection and indicate a role for CTL in protective immunity to HIV-1 in vivo.

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Topics: CTL* (59%), Cellular immunity (58%), Viremia (55%) ...read more

2,581 Citations


Open accessJournal ArticleDOI: 10.1126/SCIENCE.1178746
Laura M. Walker1, Sanjay Phogat2, Po-Ying Chan-Hui, Denise Wagner2  +19 moreInstitutions (4)
09 Oct 2009-Science
Abstract: Broadly neutralizing antibodies (bNAbs), which develop over time in some HIV-1-infected individuals, define critical epitopes for HIV vaccine design. Using a systematic approach, we have examined neutralization breadth in the sera of about 1800 HIV-1-infected individuals, primarily infected with non-clade B viruses, and have selected donors for monoclonal antibody (mAb) generation. We then used a high-throughput neutralization screen of antibody-containing culture supernatants from about 30,000 activated memory B cells from a clade A-infected African donor to isolate two potent mAbs that target a broadly neutralizing epitope. This epitope is preferentially expressed on trimeric Envelope protein and spans conserved regions of variable loops of the gp120 subunit. The results provide a framework for the design of new vaccine candidates for the elicitation of bNAb responses.

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Topics: Neutralizing antibody (62%), HIV vaccine (58%), Epitope (57%) ...read more

1,663 Citations


Journal ArticleDOI: 10.1126/SCIENCE.280.5371.1884
Richard T. Wyatt1, Joseph Sodroski1Institutions (1)
19 Jun 1998-Science
Abstract: The human immunodeficiency virus-type 1 (HIV-1) envelope glycoproteins interact with receptors on the target cell and mediate virus entry by fusing the viral and cell membranes. The structure of the envelope glycoproteins has evolved to fulfill these functions while evading the neutralizing antibody response. An understanding of the viral strategies for immune evasion should guide attempts to improve the immunogenicity of the HIV-1 envelope glycoproteins and, ultimately, aid in HIV-1 vaccine development.

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Topics: Viral envelope (65%), Viral entry (62%), HIV Antigens (52%) ...read more

1,611 Citations


Open accessJournal ArticleDOI: 10.1126/SCIENCE.1187659
Xueling Wu1, Zhi Yong Yang1, Yuxing Li1, Carl Magnus Hogerkorp1  +20 moreInstitutions (3)
13 Aug 2010-Science
Abstract: Cross-reactive neutralizing antibodies (NAbs) are found in the sera of many HIV-1-infected individuals, but the virologic basis of their neutralization remains poorly understood. We used knowledge of HIV-1 envelope structure to develop antigenically resurfaced glycoproteins specific for the structurally conserved site of initial CD4 receptor binding. These probes were used to identify sera with NAbs to the CD4-binding site (CD4bs) and to isolate individual B cells from such an HIV-1-infected donor. By expressing immunoglobulin genes from individual cells, we identified three monoclonal antibodies, including a pair of somatic variants that neutralized over 90% of circulating HIV-1 isolates. Exceptionally broad HIV-1 neutralization can be achieved with individual antibodies targeted to the functionally conserved CD4bs of glycoprotein 120, an important insight for future HIV-1 vaccine design.

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Topics: Neutralizing antibody (56%), Monoclonal antibody (54%), Viral envelope (52%) ...read more

1,582 Citations


Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20228
2021758
2020476
2019266
2018271
2017273

Top Attributes

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Topic's top 5 most impactful authors

David C. Montefiori

188 papers, 15.5K citations

John R. Mascola

83 papers, 10K citations

Dennis R. Burton

70 papers, 15.2K citations

Barney S. Graham

45 papers, 5.1K citations

Andrew B. Ward

41 papers, 3.1K citations

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