Topic
Nigella damascena
About: Nigella damascena is a research topic. Over the lifetime, 121 publications have been published within this topic receiving 1390 citations. The topic is also known as: love-in-a-mist & Devil-in-a-bush.
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TL;DR: In this article, the homology of legumin-like proteins and vicilin-like protein was demonstrated in seeds of different plant taxa. But, the similarity of these proteins was not as strong as the similarity between the two types of proteins.
16 citations
TL;DR: Callus derived from the pedicels and floral buds of Nigella damascena L. when broken down to single cells and small clumps produced large numbers of embryoids, comparable to zygotic embryogeny in a defined nutrient medium.
Abstract: Callus derived from the pedicels and floral buds of Nigella damascena L. when broken down to single cells and small clumps produced large numbers of embryoids. These embryoids passed through stages...
16 citations
TL;DR: Nigellic acid [(+)( S-7′-hydroxy-ABA], previously isolated from cell suspension cultures of Nigella damascena fed ± abscisic acid was detected as an endogenous compound in leaves of Vicia faba using gas chromatography/mass spectrometry.
16 citations
TL;DR: The main objective of this study was to develop in vitro systems utilizing N. damascena seedlings, as an easily accessible explant source, for efficient callus induction and proliferation, and plant regeneration via somatic embryogenesis, and to validate the usefulness of the obtained callus as a source of protoplasts and their capability to develop into plants.
Abstract: In this study we report the development of effective in vitro systems for a medicinal plant Nigella damascena L. comprising: (1) callus induction, (2) somatic embryogenesis in callus cultures with subsequent plant regeneration, and (3) isolation and regeneration of callus-derived protoplasts. Callus development was achieved on 83–100% of hypocotyl and cotyledon explants, whereby Murashige and Skoog medium (MS) supplemented with 3 mg L−1 6-benzylaminopurine and 0.5 mg L−1 α-naphthaleneacetic acid (NAA; BN medium) was more advantageous than MS with kinetin and NAA (KN medium). Histological observations of calli revealed the presence of embryogenic zones from which somatic embryos developed on the hormone-free medium. Plant regeneration was observed on 76–95% of calli. A high capacity to form somatic embryos and regeneration was maintained in long-lasting cultures, i.e. even in 2 year old callus. The obtained callus was also a good source tissue for protoplast isolation. By applying a mixture of cellulase and pectolyase, the acceptable yield of viable protoplasts was achieved, especially from hypocotyl-derived callus maintained on BN medium. Protoplasts embedded in an alginate matrix and cultured in modified Kao and Michayluk media re-constructed their cell wall and re-entered mitotic divisions. About 30% of small cell aggregates formed microcalli, which, after the release from alginate, proliferated continuously on KN and BN media, irrespective of the tissue variant used as the protoplast source. Somatic embryo formation and plant regeneration were successful on hormone-free media. An effective plant regeneration system of N. damascena protoplast cultures has been developed and is being reported for the first time. The main objective of this study was to develop in vitro systems utilizing N. damascena seedlings, as
an easily accessible explant source, for efficient callus induction and proliferation, and plant
regeneration via somatic embryogenesis. Moreover, we attempted to validate the usefulness of the
obtained callus as a source of protoplasts and their capability to develop into plants.
15 citations
TL;DR: A new phenolic ester has been isolated from the seeds of Nigella damascena and the structure was established as 1-O-(2,4-dihydroxy)phenylacetyl glycerol by 1H and 13C-NMR spectral data and EI-MS analysis.
15 citations