Nigericin

TL;DR: It was shown that the reconstitution of vesicular structures with functional activity required several hours and that the formation of new vesicles from solubilized P-lipids was obtained by demonstrating inclusion of macromolecules such as 14C-labeled inulin or ferritin which could not be removed by washing.

TL;DR: A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm.

TL;DR: It is suggested that ATP and nigericin induce a net decrease in intracellular levels of K+ which is necessary for activation of the post-translational maturation of IL-1 beta.

TL;DR: The results establish the application of GFP as a targetable, noninvasive indicator of intracellular pH and suggest that GFP pH sensitivity involves simple protonation events at a pH of >5, but both protonations and conformational changes at lower pH.

TL;DR: The results affirm the general utility of toxic antibiotics as tools for metabolic investigations and show considerable selectivity in these systems as well as in their inhibition of mitochondrial adenosinetriphosphatase (ATPase).