Topic
Nitrate reductase
About: Nitrate reductase is a research topic. Over the lifetime, 7659 publications have been published within this topic receiving 254531 citations. The topic is also known as: nitrite:(acceptor) oxidoreductase & nitrite:acceptor oxidoreductase.
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TL;DR: A method for simultaneous evaluation of nitrate and nitrite concentrations in a microtiter plate format is developed and S-Nitrosothiols and L-arginine derivatives were found to be potential interfering agents.
3,170 citations
TL;DR: The measurement of nitrate reductase activity in intact plant tissue based on reduction of nitrates to nitrite is facilitated by the use of propanol and other organic solvents added to the incubation medium.
1,135 citations
TL;DR: The availability of efficient nuclear and chloroplast transformation in Chlamydomonas provides specific advantages for the study of chloropleft biogenesis, photosynthesis, and nuclear-chloroplast genome interactions.
Abstract: By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, nuclear transformation rates of approximately 10(3) transformants per micrograms of plasmid DNA were achieved. The nitrate reductase gene from wild-type Chlamydomonas was used to complement a mutation in the corresponding gene of a strain containing nit1-305. Transformants were selected by growth with nitrate as sole source of nitrogen. The transforming DNA integrated into the genome at a low-copy number in nit+ transformants. When cells carrying nit1-305 were agitated in the presence of two plasmids, one with the gene for nitrate reductase and the second with an unselected gene, the unselected gene was present in 10-50% of nit+ transformants. This high frequency of cotransformation will allow any cloned gene to be introduced into Chlamydomonas. Moreover, the overall efficiency of transformation should be high enough to permit isolation of genes from genomic libraries by complementation of stable nuclear mutants. The availability of efficient nuclear and chloroplast transformation in Chlamydomonas provides specific advantages for the study of chloroplast biogenesis, photosynthesis, and nuclear-chloroplast genome interactions.
1,099 citations
TL;DR: This fluorometric method combines speed and sensitivity with the handling of a large number of samples for the quantification of nitrite generated from in vivo and in vitro sources.
1,041 citations
TL;DR: The result of experiments indicate that the synthesis of nitrate reductase is induced by nitrate and repressed by ammonium, and no effect in vitro of ammonium could be found.
1,012 citations