scispace - formally typeset
Search or ask a question
Topic

NS5B

About: NS5B is a research topic. Over the lifetime, 1314 publications have been published within this topic receiving 59534 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: The data suggest that HCV-TR1 encodes functionally intact viral proteins, but it also encodes altered viral epitopes, which may affect host immune-response.
Abstract: Based on direct sequencing information from 5′UTR and NS5B regions, we identified subtype 1b as a predominant hepatitis C virus genome in Turkey, which affected more than 91% of 79 patients studied. Next, the full genome sequence of a Turkish 1b isolate was obtained by the cloning of polypeptide-encoding region into 7 overlapping fragments. Turkish 1b isolate, which was named HCV-TR1, comprises 9361 nucleotides, including 306 nucleotides of 5′UTR, a single long open reading frame of 9033 nucleotides, and 22 nucleotides of 3′UTR. When compared to HCV 1b polypeptide sequences available at GenBank, the predicted polypeptide displayed a total of 36 amino acid substitutions, of which 16 was specific for HCV-TR1 isolate. Despite these changes, major structural and functional motifs of HCV proteins were maintained in HCV-TR1. In contrast, HCV-TR1 displayed amino acid substitutions in 6 out of 9 major cytotoxic T-cell epitopes. These data suggest that HCV-TR1 encodes functionally intact viral proteins, but it also encodes altered viral epitopes, which may affect host immune-response.

24 citations

Journal ArticleDOI
TL;DR: A homogeneous, solid-phase, high-throughput biochemical assay for detecting NS5B enzymatic activity and offering the advantage of delaying initiation, mimicking a physical preincubation step required for evaluating time-dependent inhibitors.

24 citations

Journal ArticleDOI
Guanghui Yi1, Chu-Yu Zhang1, Sheng Cao1, Haixiang Wu1, Yi Wang1 
TL;DR: De novo RNA synthesis represented the preferred mechanism for initiation of classical swine fever virus RNA synthesis by RNA-dependent RNA polymerase in vitro, and both Mg2+ and Mn2+ supported de novo initiation, however, RNA synthesis was more efficient in the presence of Mn2+.
Abstract: Classical swine fever virus nonstructural protein 5B (NS5B) encodes an RNA-dependent RNA polymerase, a key enzyme of the viral replication complex. To better understand the initiation of viral RNA synthesis and to establish an in vitro replication system, a recombinant NS5B protein, lacking the C-terminal 24-amino acid hydrophobic domain, was expressed in Escherichia coli. The truncated fusion protein (NS5Bdelta24) was purified on a Ni-chelating HisTrap affinity column and demonstrated to initiate either plus- or minus-strand viral RNA synthesis de novo in a primer-independent manner but not by terminal nucleotidyle transferase activity. De novo RNA synthesis represented the preferred mechanism for initiation of classical swine fever virus RNA synthesis by RNA-dependent RNA polymerase in vitro. Both Mg2+ and Mn2+ supported de novo initiation, however, RNA synthesis was more efficient in the presence of Mn2+ than in the presence of Mg2+. De novo initiation of RNA synthesis was stimulated by preincubation with 0.5 mm GTP, and a 3'-terminal cytidylate on the viral RNA template was preferred for de novo initiation. Furthermore, the purified protein was also shown, by North-Western blot analysis, to specifically interact with the 3'-end of both plus- and minus-strand viral RNA templates.

24 citations

Journal ArticleDOI
13 Apr 2010-PLOS ONE
TL;DR: In this paper, the authors developed and evaluated a real-time PCR method for hepatitis C virus genotyping based on the NS5B region, which is the most significant predictor of the response to antiviral therapy.
Abstract: Background Hepatitis C virus (HCV) genotyping is the most significant predictor of the response to antiviral therapy. The aim of this study was to develop and evaluate a novel real-time PCR method for HCV genotyping based on the NS5B region.

23 citations

Journal ArticleDOI
TL;DR: The impact of the L30S variant on the inhibition profile and binding kinetics of BMS-791325 provides experimental evidence for the dynamic interaction of fingers and thumb domains in an environment that supports the formation of active replication complexes and the initiation of RNA synthesis.

23 citations


Network Information
Related Topics (5)
Hepatitis C virus
32.2K papers, 1.1M citations
88% related
Viral replication
33.4K papers, 1.6M citations
86% related
Hepatitis B virus
39.1K papers, 1.2M citations
85% related
Interferon
28.9K papers, 1.2M citations
83% related
Virus
136.9K papers, 5.2M citations
82% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202310
202258
202128
202033
201943
201842