Topic
Nuclear DNA
About: Nuclear DNA is a research topic. Over the lifetime, 3933 publications have been published within this topic receiving 185830 citations.
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TL;DR: The chapter focuses on the use of 6-diamidino-2-phenylindole (DAPI) for flow cytometry (FCM), which proved to be a specific, highly fluorescent stain, very well suited for FCM of DNA in whole cells, nuclei, and chromosomes.
Abstract: Publisher Summary Protocols are available for high-resolution analysis of nuclear DNA that enable to discriminate somatic cells from male and female donors and to characterize precisely the ploidy state of tumors This chapter discusses protocols developed for use in cells obtained from cell suspensions and solid tissues. The procedures include fixation with 70% ethanol. Thus, they are applicable in specimens that have to be stored before measurement. The chapter focuses on the use of 6-diamidino-2-phenylindole (DAPI) for flow cytometry (FCM). DAPI proved to be a specific, highly fluorescent stain, very well suited for FCM of DNA in whole cells, nuclei, and chromosomes. The protocols are recommended for accurate and reproducible measurements of the nuclear DNA, especially for the assessment of DNA content variations in cell populations affected by chemical or physical mutagens and for the reliable detection and characterization of aneuploid cell clones in tumors.
87 citations
TL;DR: Modulation by rutin of these parameters emphasizes the protective role of this flavonoid against carcinogenesis induced by chemical carcinogens.
87 citations
TL;DR: The sedimentation behavior of high molecular weight nuclear and mitochondrial DNA from the yeast Saccharomyces cerevisiae has been examined and the molecular weights estimated based on a linear, double-stranded configuration are estimated.
87 citations
TL;DR: A homolog of cdc2 from Aspergillus nidulans that can complement the Schizosaccharomyces pombe cDC2-33 mutation is cloned and sequenced and designated nimXcdc2 as it is not allelic to any of the other nim genes (nimA to nimW) of A. ndulans.
Abstract: We have cloned and sequenced a homolog of cdc2 from Aspergillus nidulans that can complement the Schizosaccharomyces pombe cdc2-33 mutation. The gene was deleted and is required for continued nuclear DNA replication but not for mitochondrial DNA replication. Three different temperature-sensitive alleles were generated by reverse genetics. All of the mutations generate the nim phenotype of A. nidulans. The new gene was designated nimXcdc2 as it is not allelic to any of the other nim genes (nimA to nimW) of A. nidulans. Reciprocal shift experiments place an essential function for nimXcdc2 in G1 and G2. Antipeptide antibodies were generated that detect NIMXcdc2, and antisera were also generated to detect NIMEcyclinB. The two p34cdc2 protein species previously detected in A. nidulans, p34 and p37, both precipitate using NIMXcdc2 C-terminus-specific antibodies but only p34 co-precipitates with NIMEcyclinB. Dephosphorylation of denatured p34 converts it to the p37 form, showing p37 to be the non-phosphorylated form of NIMXcdc2. The phosphorylation of p34 is therefore associated with its interaction with NIMEcyclinB.
86 citations
TL;DR: This survey, using several restriction enzymes, showed that Sma I digestions of total DNA could be used to differentiate these two closely related species of Aspergillus.
86 citations