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Nuclear DNA

About: Nuclear DNA is a research topic. Over the lifetime, 3933 publications have been published within this topic receiving 185830 citations.


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Journal ArticleDOI
01 Feb 1976-Heredity
TL;DR: There is wide variation in chromosome size and nuclear DNA amount between the 22 species studied within the genus Crepis, with the most primitive perennial species having higher DNA values than the more advanced annual species.
Abstract: There is wide variation in chromosome size and nuclear DNA amount between the 22 species studied within the genus Crepis. The changes in DNA amount involve all, or most of, the chromosomes within species complements, but structural rearrangements due to inversions and interchanges have made the distribution of changes somewhat unequal. There is an evolutionary reduction in the amount of DNA with the most primitive perennial species having higher DNA values than the more advanced annual species. The advanced species have more chromosomes with median centromeres than the primitive species and show increased symmetry. Nuclear DNA amount is positively correlated with both seed size and pollen grain size.

73 citations

Journal ArticleDOI
TL;DR: With further optimization, this method could provide a basic evaluation of minimum primate dietary diversity even when knowledge of local flora is limited and may find application in studies characterizing the diets of poorly‐known, unhabituated primate species or assaying consumer–resource relationships in an ecosystem.
Abstract: Analyses of plant DNA in feces provides a promising, yet largely unexplored, means of documenting the diets of elusive primates. Here we demonstrate the promise and pitfalls of this approach using DNA extracted from fecal samples of wild western gorillas (Gorilla gorilla) and black and white colobus monkeys (Colobus guereza). From these DNA extracts we amplified, cloned, and sequenced small segments of chloroplast DNA (part of the rbcL gene) and plant nuclear DNA (ITS-2). The obtained sequences were compared to sequences generated from known plant samples and to those in GenBank to identify plant taxa in the feces. With further optimization, this method could provide a basic evaluation of minimum primate dietary diversity even when knowledge of local flora is limited. This approach may find application in studies characterizing the diets of poorly-known, unhabituated primate species or assaying consumer-resource relationships in an ecosystem.

73 citations

Journal ArticleDOI
TL;DR: The levels of endogenous pig liver cells mitochondrial DNA oxidative base damage have been investigated using isotope dilution gas chromatography mass spectrometry (GC/MS), and large differences in the modified base ratios of mitochondrial versus nuclear DNA are detected.

73 citations

Journal ArticleDOI
TL;DR: The identification of causative molecular defects in nuclear or mitochondrial genome leads to the definite diagnosis of mitochondrial myopathy, which is characterized by early fatigue and/or fixed muscle weakness.

73 citations

Journal ArticleDOI
TL;DR: Gossypium gossypioides provides a striking example of the previously unsuspected chimeric nature of some plant genomes and the resulting phylogenetic complexity produced by multiple historical reticulation events.
Abstract: The Mexican cotton Gossypium gossypioidesis a perplexing entity, with conflicting morphological, cyto- genetic, and molecular evidence of its phylogenetic affinity to other American cottons. We reevaluated the evolutionary history of this enigmatic species using 16.4 kb of DNA sequence. Phylogenetic analyses show that chloroplast DNA (7.3 kb), nuclear ribosomal internal transcribed spacers (ITS; 0.69 kb), and unique nuclear genes (8.4 kb) yield conflicting resolutions for G. gossypioides. Eight low-copy nuclear genes provide a nearly unanimous resolution of G. gossypioides as the basalmost American diploid cotton, whereas cpDNA sequences resolve G. gossypioides deeply nested within the American diploid clade sister to Peruvian G. raimondii, and ITS places G. gossypioides in an African (rather than an American) clade. These data, in conjunction with previous evidence from the repetitive fraction of the genome, implicate a complex history for G. gossypioides possibly involving temporally separated introgression events from genetically divergent cottons that are presently restricted to different hemispheres. Based on repetitive nuclear DNA, it appears that G. gossypioidesexperienced nuclear introgression from an African species shortly after divergence from the remainder of the American assemblage. More recently, hybridization with a Mexican species may have resulted in cpDNA introgression, and possibly a second round of cryptic nuclear introgression. Gossypium gossypioides provides a striking example of the previously unsuspected chimeric nature of some plant genomes and the resulting phylogenetic complexity produced by multiple historical reticulation events.

73 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202361
202284
202177
202064
201966
201862