Topic
Nuclear DNA
About: Nuclear DNA is a research topic. Over the lifetime, 3933 publications have been published within this topic receiving 185830 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: The renaturation kinetics of nuclear DNA from the G3 colony of Anopheles gambiae Giles was studied to estimate the genome size and to determine the proportion of repeated sequences.
Abstract: The renaturation kinetics of nuclear DNA from the G3 colony of Anopheles gambiae Giles was studied to estimate the genome size and to determine the proportion of repeated sequences. An. gambiae has a haploid DNA content of 0.27 picograms or 2.6 x 10(8) basepairs. Analysis of reassociation kinetics indicated that the genome is composed of 61% single-copy and 33% repetitive sequences, with 6% foldback sequences.
62 citations
TL;DR: The chromosome number of the hybrids could be determined with an accuracy of ±10% by using flow cytometry, and the smallest statistically significant difference in DNA content between two individuals was 0.23 pg DNA/cell.
Abstract: Fourty-one somatic hybrids from two species combinations, Brassica oleracea + B campestris and B napus + Eruca sativa, were analysed for chromosome number and nuclear DNA content The DNA content was measured in a flow cytometer using two internal standards as references and when related to the chromosome number a correlation of 091 was found The chromosome number of the hybrids could be determined with an accuracy of ±10% by using flow cytometry, and the smallest statistically significant difference in DNA content between two individuals was 023 pg DNA/cell
62 citations
TL;DR: It is estimated that 10 to 35% of the mitochondrial DNA replicates during oxygen adaptation, and a respiratory-deficient mutant showed a similar response to oxygen and glucose with respect to DNA synthesis although oxidative enzyme activity was absent.
61 citations
TL;DR: Electron microscopic analysis indicates that yeast nuclear DNA can be isolated as linear molecules ranging in size from 50 mum (1.2 x 10(8) daltons) to 355 mum (8.4 x 10 (8)Daltons), consistent with the hypothesis that each yeast chromosome contains a single, linear DNA duplex.
Abstract: Electron microscopic analysis indicates that yeast nuclear DNA can be isolated as linear molecules ranging in size from 50 μm (1.2 × 108 daltons) to 355 μm (8.4 × 108 daltons). Analysis indicates the data is consistent with the hypothesis that each yeast chromosome contains a single, linear DNA duplex. Mitochondrial DNA molecules have a contour length of 21 ± 2 μm and are mostly linear.
61 citations
TL;DR: Intraspecific studies suggest a connection between repetitive DNA and quantitative genetic determination of continuous characters and novel insights into mechanisms by which repetitive DNA influences phenotype will lead to models of evolutionary change that extend well beyond the conventional view of evolution by allelic substitution.
Abstract: The discovery that nuclear DNA content varies widely among species, and even within species, was unexpected because it was thought that the number of genes required for an organism should be common across taxa. We now know that the bulk of nuclear DNA content variation is caused by repetitive DNA sequences characterized according to the nature of repeat (tandem vs dispersed) or chromosomal location/mechanism of replication (pericentromeric, telomeric or subtelomeric, microsatellites, minisatellites, satellites, transposable elements, retroelements). Variation in repetitive DNA, manifested as variation in nuclear DNA content, has been shown to have broad ecological and life-history consequences. For example, large genome size appears to limit fitness in extreme environmental conditions. Within species, variation in DNA content has been coupled to growth and development, such as maturation time in crop species. In Silene latifolia, DNA content is negatively correlated with flower size, a character that, in turn, has well documented ecological significance. These intraspecific studies suggest a connection between repetitive DNA and quantitative genetic determination of continuous characters. Novel insights into mechanisms by which repetitive DNA influences phenotype will lead to models of evolutionary change that extend well beyond the conventional view of evolution by allelic substitution.
61 citations