Topic
Nuclear DNA
About: Nuclear DNA is a research topic. Over the lifetime, 3933 publications have been published within this topic receiving 185830 citations.
Papers published on a yearly basis
Papers
More filters
TL;DR: The finding that the T-DNA found in different tumor tissues is of a fixed size poses the question of whether T- DNA integrates into plant DNA via a mechanism similar to that by which transposable elements integrate into foreign DNA.
Abstract: Publisher Summary The most important characteristic that immediately distinguishes agrobacteria from most other bacteria is their ability to induce tumor formation in plants. Agrobacteria transform “conditioned” plant cells into tumor cells by introducing into them a piece of genetic information which in established plant tumor cells can be recovered as T-DNA integrated into the nuclear plant DNA. Tumor cells characteristically contain a set of unusual amino acid derivatives that have never been found in normal plant cells. Crown gall cells carry a piece of DNA that is homologous to a part of A. turnefuciens DNA integrated into their nuclear DNA. The T-DNA is responsible for the phytohormone-independent growth of tumor cells and encodes enzymes that synthesize opines in the tumor cells. Moreover, the finding that the T-DNA found in different tumor tissues is of a fixed size poses the question of whether T-DNA integrates into plant DNA via a mechanism similar to that by which transposable elements integrate into foreign DNA. The answer to this question is not known. However, it is known that the ends of the T-region are important for tumor induction.
128 citations
TL;DR: A procedure is presented for the rapid isolation of mitochondrial DNA (mtDNA) in high yield from Saccharomyces cerevisiae by a single cycle of bisbenzimide-CsCl buoyant density centrifugation.
128 citations
TL;DR: The results show that moa were characterized by extreme reverse sexual dimorphism and as a result they have been able to clarify the number of moa species and illustrates that single-locus nuclear DNA sequences can be consistently recovered from ancient material.
Abstract: Ancient DNA studies have typically used multi-copy mitochondrial DNA sequences1,2. This is largely because single-locus nuclear genes have been difficult to recover from sub-fossil material3, restricting the scope of ancient DNA research. Here, we have isolated single-locus nuclear DNA markers to assign the sex of 115 extinct moa and, in combination with a mitochondrial DNA phylogeny, tested competing hypotheses about the specific status of moa taxa. Moa were large ratite birds that showed extreme size variation both within and among species4. For some taxa, this large variation was hypothesized to represent sexual dimorphism, while for others it was argued to reflect the existence of different species5. Our results show that moa were characterized by extreme reverse sexual dimorphism and as a result we have been able to clarify the number of moa species. For example, we show that the three recognized ‘species’ of Dinornis comprised only two monophyletic groups and that two of these ‘species’ comprised individuals of one sex only. This study also illustrates that single-locus nuclear DNA sequences can be consistently recovered from ancient material.
128 citations
TL;DR: Nuclei isolated from rat liver in the absence of Ca2+ incorporate deoxyribonucleotides into added poly d(A-T) but not into their nuclear DNA, which is of high molecular weight.
128 citations
TL;DR: A loss of this function most likely results in a defect in DNA replication, leading directly, or via the activation of a DNA damage checkpoint, to an arrest of cell division and endoreduplication.
127 citations