Nucleic acid methods

About: Nucleic acid methods is a research topic. Over the lifetime, 4328 publications have been published within this topic receiving 137473 citations.

Process for amplifying nucleic acid sequences

Abstract: The present invention is directed to a process for amplifying any desired specific nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, and extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired.

Nucleic acid techniques in bacterial systematics

Abstract: Isolation and purification of nucleic acids DNA reassociation experiments DNA-rRNA hybridization and methods DNA sequencing in bacterial systematics direct sequence analysis of small RNAs 16S/23S rRNA sequencing the polymerase chain reaction development and application of nucleic acid probes DNA fingerprinting from macromolecules to trees.

[99] Determination of nucleic acids in tissues by pentose analysis

Abstract: Publisher Summary The procedure for the determination of nucleic acids described in this chapter is based on the finding that nucleic acids can be separated from other tissue compounds by their preferential solubility in hot trichloroacetie acid The isolated nucleic acids are then quantitated by means of colorimetric reactions involving the pentose components of the nucleic acids The determination of nucleic acids in tissues is largely a problem in identification By means of the extraction procedures described in the chapter and the colorimetric reactions of peptide nucleic acid and DNA, a considerable degree of specificity is placed on the determination of these compounds Occasionally however, false results will be obtained, owing to the presence of materials in the nucleic acid extracts that interfere with the pentose reactions It is emphasized that the extraction methods described were developed for nucleic acid determinations by spectrophotometric methods Although it was at first thought that these procedures might be directly applicable to isotopic work, it has become quite clear that the separations are not sufficiently refined for such studies The methods have served, however, as starting points for other separation procedures more suitable for isotopic work