Topic
Nucleolar chromatin
About: Nucleolar chromatin is a research topic. Over the lifetime, 170 publications have been published within this topic receiving 6776 citations.
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TL;DR: This work considers this structure observed in the nucleolus of Allium cepa meristematic cells after nucleolar disgregation, by a continuous treatment of 12 h with ethidium bromide, as the intranucleolar chromatin collapsed by the effect of the drug.
Abstract: This work is an ultrastructural and cytochemical study of a structure observed in the nucleolus of Allium cepa meristematic cells after nucleolar disgregation, by a continuous treatment of 12 h with ethidium bromide. The ultrastructural and cytochemical data allow us to consider this structure as the intranucleolar chromatin collapsed by the effect of the drug.
1 citations
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01 Jan 1990
TL;DR: The goal of this study was to examine the amplified nucleoli by light and electron microscopy and to describe the organization of their transcriptionally active chromatin.
Abstract: The structure of transcriptionally active chromatin of amplified oocyte nucleoli of amphibians has been studied in detail (Miller, Beatty, 1969; Scheer, Zentgraf, 1982). It has been shown that tandem arrays of transcription units (TUs) are the basic structural elements transcribed in chromatin of nucleoli. However, light microscope data on the structure of nucleoli during oogenesis give some grounds to assume that transcriptionally active chromatin of nucleoli is organized into a higher order structure, as compared to the linearly arranged TUs. For instance, in vitellogenic amphibian oocytes, the spherical nucleoli acquire the appearance of bead-string rings (Callan, 1966). Ring structures with beads along the axis have been found directly in the body of spherical nucleoli during examination of thin section oocyte nuclei (Parfenov, 1983). The question of the correspondence of structures observed by different methods of the investigation is still to be answered. The goal of this study was to examine the amplified nucleoli by light and electron microscopy and to describe the organization of their transcriptionally active chromatin.
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TL;DR: In this paper, the authors demonstrated that PHF6 can bind to H3K9me3 and H3k27me1 on the nucleolar chromatin and recruit histone methyltransferase SUV39H1 to the rDNA locus.
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01 Jan 1981TL;DR: One useful approach to explore the molecular background of various observed end-effects is to study the effect of damage on a specific gene which has been isolated in its chromatin form.
Abstract: Chromatin is an important target for the effect of chemicals and ionizing radiation in living cells The structural alterations induced lead to disturbance of the genetic expression, ie of the biosynthesis of RNA and of protein One useful approach to explore the molecular background of various observed end-effects is to study the effect of damage on a specific gene which has been isolated in its chromatin form
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TL;DR: Possible functional implications of the chromosomal proteins are discussed and the electrophoretic properties of the histones, nonhistone proteins and ribonucleoprotein particles were examined.