Topic
ORC1
About: ORC1 is a research topic. Over the lifetime, 147 publications have been published within this topic receiving 10296 citations. The topic is also known as: HSORC1 & ORC1L.
Papers published on a yearly basis
Papers
More filters
TL;DR: The data suggest that the loading of Mcm proteins onto origins is regulated by two mechanisms: first, by Cdc6p occupancy, and second, by S- and M-CDKs, whose activity during S, G2, and M phases prevents Mcm loading.
522 citations
TL;DR: It is shown that TERRA RNA can interact directly with the TRF2 GAR and ORC1 to form a stable ternary complex and plays a central role in telomere structural maintenance and heterochromatin formation.
486 citations
TL;DR: Matches between its predicted amino acid sequence and peptide sequence obtained from the 50-kilodalton subunit of the yeast origin recognition complex (ORC) established that the gene isolated here, ORC6, encodes this subunit, providing evidence that ORC recognizes yeast replication origins in vivo.
Abstract: Here a method is described to identify genes encoding proteins that recognize a specific DNA sequence. A bank of random protein segments tagged with a transcriptional activation domain is screened for proteins that can activate a reporter gene containing the sequence in its promoter. This strategy was used to identify an essential protein that interacts in vivo with the yeast origin of DNA replication. Matches between its predicted amino acid sequence and peptide sequence obtained from the 50-kilodalton subunit of the yeast origin recognition complex (ORC) established that the gene isolated here, ORC6, encodes this subunit. These observations provide evidence that ORC recognizes yeast replication origins in vivo.
446 citations
TL;DR: Characterization of a temperature-sensitive mutation in the gene encoding the 72-kD subunit of ORC (ORC2) indicates that this protein complex functions early in the DNA replication process, and supports the hypothesis that ORC acts as an initiator protein at yeast origins of DNA replication and suggests that OrC also functions in the determination of transcriptional domains.
Abstract: The genes encoding two of the subunits of the Saccharomyces cerevisiae origin recognition complex (ORC) have been isolated. Characterization of a temperature-sensitive mutation in the gene encoding the 72-kD subunit of ORC (ORC2) indicates that this protein complex functions early in the DNA replication process. Moreover, ORC derived from orc2ts cells is defective for DNA binding. Others have shown a defect in orc2ts cells in transcriptional silencing at the silent mating-type loci. Consistent with this finding, ORC specifically binds to each of the four mating-type silencers identified in yeast. These findings support the hypothesis that ORC acts as an initiator protein at yeast origins of DNA replication and suggest that ORC also functions in the determination of transcriptional domains.
439 citations
TL;DR: Roles for ORC-mediated acetylation of chromatin in control of both DNA replication and gene expression are suggested.
330 citations