scispace - formally typeset
Search or ask a question
Topic

Paper chromatography

About: Paper chromatography is a research topic. Over the lifetime, 3240 publications have been published within this topic receiving 65381 citations. The topic is also known as: Chromatography paper.


Papers
More filters
Journal ArticleDOI
TL;DR: Paper chromatography has been used in the identification of compounds formed by plants during short periods of photosynthesis as mentioned in this paper, and it has been shown to be useful in the separation of polycarboxylic acids and phosphate esters.
Abstract: Investigation of the reduction of carbon dioxide in plants requires a rapid and general method for separating and identifying a large number of compounds. Paper chromatography is such a method. This paper constitutes a report of progress in the development of experimental procedures in the application of paper chromatography to the separation of compounds formed by plants during short periods of photosynthesis. The compounds involved are important intermediates of plant and animal metabolism. These methods should find application not only in other biochemical problems, but in the control of chemical synthesis2 as well. A number of products formed during a short period of photosynthesis with radioactive carbon dioxide have been r e p ~ r t e d . ~ ~ * ~ ~ , ~ ~ ~ The classical biochemical methods and ion exchange techniques which were used in those identifications would require considerable effort to separate the large number of compounds involved. The success of paper chromatography in separating two groups of compounds important in phytosynthesis, amino acidss and s u g ~ t r s , ~ ~ ~ ~ prompted the present work on separation of polycarboxylic acids and phosphate esters. These compounds constitute the presently known intermediates in synthesis of plant material. l1 The primary requisite for the use of this method is some means of determining the position of a compound in the chromatogram. Both chemical and physical methods have been used. The former includes color tests which depend on development of a color directly on the paper by the use of specific reagents: ninhydrin for amino acids,*naphthoresorcinolor Tollens reagent for sugars,’” ferric ion for hydroxamic acid^,'^^^^ $H in-

464 citations

Journal ArticleDOI
TL;DR: It is concluded that a point mutation in the human ALDH2 locus produced the glutamine leads to lysine substitution and enzyme inactivation in atypical livers.
Abstract: Usual human livers contain two major aldehyde dehydrogenase [(ALDH) aldehyde:NAD+ oxidoreductase] isozymes--i.e., a cytosolic ALDH1 component and a mitochondrial ALDH2 component--whereas approximately equal to 50% of Orientals are "atypical" and have only the ALDH1 isozyme and are missing the ALDH2 isozyme. We previously demonstrated that atypical livers contain an enzymatically inactive but immunologically crossreactive material (CRM) corresponding to the ALDH2 component. The enzymatically active ALDH2 obtained from a usual liver and the CRM obtained from an atypical liver were reduced, S-carboxymethylated, and digested by trypsin. Separation of their digests by high-performance reverse-phase chromatography and by two-dimensional paper chromatography and electrophoresis revealed that ALDH2 contained a peptide sequence of -Glu-Leu-Gly-Glu-Ala-Gly-Leu-Gln-Ala-Asn-Val-Gln-Val-Lys- and that the glutamine adjacent to lysine was substituted by lysine in CRM. All other tryptic peptides, including eight peptides containing S-carboxymethylcysteine, were common in ALDH2 and CRM. It is concluded that a point mutation in the human ALDH2 locus produced the glutamine leads to lysine substitution and enzyme inactivation.

421 citations

Journal ArticleDOI
TL;DR: Radioactively labeled S-methylcysteine has been identified in protein hydrolysates after incubation of the alkylated DNA with a partly purified E. coli methyltransferase activity.

412 citations

Journal ArticleDOI
TL;DR: Alpha-amylase (EC 3.1.2.1), which cleaves internal -1,4glycosidic linkages in starch to produce glucose, maltose, or dextrins, and glucoamylases (EC 4.2) are widely used in the industrial conversion of starch into sugars.

411 citations


Network Information
Related Topics (5)
Amino acid
124.9K papers, 4M citations
81% related
High-performance liquid chromatography
47.3K papers, 1M citations
81% related
Enzyme
32.8K papers, 1.1M citations
81% related
Metabolism
19.3K papers, 761.5K citations
80% related
Polyacrylamide gel electrophoresis
17.4K papers, 693.3K citations
80% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
20212
20205
20191
20185
20178