Showing papers on "Penicillin amidase published in 1970"
TL;DR: Results of techniques involving the reaction of products formed with iodine, hydroxylamine, and phenylacetylchloride indicate the presence of β-lactamase in purified enzyme preparations, and penicilloic acid appears to be the only product formed during the reaction.
4 citations
Patent•
04 Mar 1970
TL;DR: In this paper, an organic polymer, e.g. cellulose, is substituted by groups of the formula where X represents a radical capable of reacting with a biologically active substance and Y represents a halogen atom or a nucleophilic amino, aromatic or aliphatic group.
Abstract: 1,183,259. Triazinyl substituted polymers; enzymes. NATIONAL RESEARCH DEVELOPMENT CORP. 2 July, 1968 [14 July, 1967; 12 Feb., 1968; 10 April, 1968], Nos. 32541/67, 6869/68 and 17322/68. Headings C3A and C3H. [Also in Division Dl] . _ An organic polymer, e.g. cellulose, which is substituted (i) by groups of the formula where X represents a radical capable of reacting with a biologically active substance and Y represents a halogen atom or a nucleophilic amino, aromatic or aliphatic group; and (ii) by a further substituent group which bears a positive charge when in contact with a solution having a pH in the normal biological range, is made by contacting the polymer already containing the group (ii) with a triazine bearing N-substituents X and Y as defined and a further N-substituent Z which is a halogen. Many groups Y and (ii) are listed. In examples diethylaminoethyl cellulose is reacted under alkaline conditions with (1) and (2), 2,4-dichloro-6-carboxymethylamino - s - triazine; or (3) an orange dye which contains a suitably substituted s-triazinyl group. , Enzymes may be reacted with these products at the group X. The products of the examples arc reacted under alkaline conditions with (1) [3- galactosidase, (2) penicillin amidase, (3) lactic dehydrogenase. The reaction product with (2) is used to convert benzyl penicillin to 6-aminopenicillinanic acid.
1 citations
Patent•
25 Nov 1970
TL;DR: In this paper, the authors present a novel leafage process for the production of 6-AMINOPENICILLANIC ACID, which is a 6-amino acid that is capable of contacting a PENICLLIN of the formula: 6-(R-CO-NH-),2-(HOOC-),3,3-DI(CH3-)PENAM wherein R is PHENOXYMETHYL, P-CRESOXYmETHyl or NBUTOXY mETHyl, OR an AL
Abstract: THE INVENTION CONCERNS A NOVEL CLEAVAGE PROCESS FOR THE PRODUCTION OF 6-AMINOPENICILLANIC ACID, WHICH COMPRISES CONTACTING A PENICILLIN OF THE FORMULA: 6-(R-CO-NH-),2-(HOOC-),3,3-DI(CH3-)PENAM WHEREIN R IS PHENOXYMETHYL, P-CRESOXYMETHYL OR NBUTOXYMETHYL, OR AN ALKALI METAL SALT OF SAID PENICILLIN, WITH PENICILLIN AMIDASE PRODUCED BY CULTURING UNDER AEROBIC CONDITIONS BOVISTA PLUMBEA (NNRL 3501) OR A MUTANT OR VARIANT THEREOF IN AN AQUEOUS MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN.
1 citations