Topic
Penicillin amidase
About: Penicillin amidase is a research topic. Over the lifetime, 576 publications have been published within this topic receiving 15563 citations. The topic is also known as: penicillin amidohydrolase & ampicillin acylase.
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34 citations
TL;DR: Stabilised derivatives were found to be less inhibited by the nucleophile than non-stabilised ones, improving in that way the reaction rate and more resistance under unfavourable conditions (e.g. presence of methanol) was found using stabilised derivatives.
Abstract: The advantages of the use of stabilised derivatives in the kinetically controlled synthesis catalysed by penicillin G acylase has been discussed. Stabilised derivatives were found to be less inhibited by the nucleophile than non-stabilised ones, improving in that way the reaction rate. Also, more resistance under unfavourable conditions (e.g. presence of methanol) was found using stabilised derivatives. Multipoint covalently attached derivatives prepared in the presence of penicillin sulfoxide showed the same hydrolytic/synthetic ratio as the non-distorted enzyme suggesting that the stabilisation strategy is able to increase the enzyme rigidity without promoting significant changes in the enzyme properties. In addition, the effect of the enzyme source was found to be significant: enzyme derivatives from Escherichia coli appeared to have better properties as catalysts of these reactions than enzyme derivatives of penicillin G acylase from Kluivera citrophila .
34 citations
TL;DR: Macroporous beaded polymers of varying pore sizes were synthesized coated with polyethyleneimine and derivatized with glutaraldehyde to generate aldehyde pendant groups and immobilization enhanced the thermal stability of the enzyme.
34 citations
TL;DR: The best carriers for penicillin acylase immobilization are crosslinked with DEGDA as they display preferential sorption and then immobilization of Penicillin Acylase, what leads to highly active preparations.
33 citations
TL;DR: The Bacillus sphaericus gene encoding penicillin V amidase, which catalyzes the hydrolysis of penicillus V, has been characterized and high expression of the gene was obtained in Escherichia coli using an inducible promoter, showing that the gene product is stable in this heterologous host.
33 citations