Topic
Penicillin amidase
About: Penicillin amidase is a research topic. Over the lifetime, 576 publications have been published within this topic receiving 15563 citations. The topic is also known as: penicillin amidohydrolase & ampicillin acylase.
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TL;DR: In this paper, N 6 -Phenylacetyl-2′-deoxyadenosine and N 2 -phenyl-acetyl 2′deoxyguanosine are readily deprotected in reactions catalyzed by free and immobilized penicillin amidase at pH 7.8 and 25°C.
19 citations
TL;DR: Azlactone-functional dispersion and reverse phase suspension polymer supports were examined as immobilizing media for Penicillin G Acylase (PGA) in this paper, and the most effective supports were those that also contained primary and/or secondary amide functional groups.
Abstract: Azlactone-functional dispersion and reverse phase suspension polymer supports were examined as immobilizing media for Penicillin G Acylase (PGA). Results indicated that the most effective supports were those that also contained primary and/or secondary amide functional groups. A combination of hydrophobic interactions and hydrogen bonding between amide groups on the support and enzyme was proposed to provide important and intimate association between PGA and support prior to covalent coupling. Relative to an oxirane-functional commercial standard, the azlactone biocatalyst supports featured shorter coupling times and higher catalytic activities.
19 citations
TL;DR: The results indicated that the enzyme was optimally produced with 45 g/l of dextrin, and about 43,385 and 79,880 U/l for the highest enzyme activities were obtained in batch cultivations of shaken flasks and a 3.7 l bioreactor, respectively.
19 citations
TL;DR: Poly(vinyl acetate-co-diviyl benzene) beads were synthesized via a novel polymerization method and penicillin acylase immobilized with SESA exhibited better stability than that with other activators.
Abstract: Poly(vinyl acetate-co-diviyl benzene) beads were synthesized via a novel polymerization method. The amount of hydroxide radical could reach 11.5 mmol g(-1) (wet) after the beads were catalyzed by NaOH in methanol at 18-20 degreesC for 24 h. The activators such as SESA, glutaraldehyde and p-benzoquinone were used to immobilize penicillin acylase. The penicillin acylase immobilized with SESA exhibited better stability than that with other activators. The successively immobilization could increase the activity of immobilized penicillin acylase about two times and the activity yield 10%, respectively, At the optimal conditions of immobilization, the activity of immobilized penicillin acylase amounted to 812-1200 unit per gram (wet). The kinetics of immobilized penicillin acylase was also studied. The reaction constants, K,, K-iPAA K-iAPA and K-s for immobilized penicillin acylase were equal to 0.033, 0.013, 0.009 and 0.081 mmol/ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.
19 citations
TL;DR: It appeared that although both gave good results for recovery and activity, NH4Cl was a more selective eluent with a higher fold purification than imidazole, which greatly increased the degree of purification.
19 citations