Showing papers on "Penicillium griseofulvum published in 1971"
TL;DR: The properties of an amino acid accumulating system were investigated in Penicillium griseofulvum Dierckx by following the uptake of l -leucine by mycelium in the absence of external glucose, and 98% of the leucine accumulated was shown to be in unbound form in the cell.
Abstract: The properties of an amino acid accumulating system were investigated in Penicillium griseofulvum Dierckx by following the uptake of l -leucine (and other amino acids) by mycelium in the absence of external glucose. In these conditions 98% of the leucine accumulated was shown to be in unbound form in the cell. The kinetics of uptake at 25 °C may be described by a Michaelis—Menten relation with an apparent Km of 2·22 × 10−3 m . Uptake of leucine by the mycelium was abolished by several metabolic inhibitors, and the amount accumulated was markedly dependent on the pH of the medium, on the age of the mycelium, and on the source of nitrogen on which the mycelium had previously been growing. Studies of simultaneous uptake of several amino acids suggested the presence of more than one mechanism of uptake with broad but differing specificities.
7 citations
7 citations