Showing papers on "Penicillium griseofulvum published in 2019"
TL;DR: The aims of this study were to investigate the biocontrol effects of Penicillium griseofulvum strain CF3 and its mechanisms against soil‐borne root pathogens (Fusarium oxysporum and Sclerotium rolfsii) of the medical plant Aconitum carmichaelii Debx.
Abstract: Aims The aims of this study were to investigate the biocontrol effects of Penicillium griseofulvum strain CF3 and its mechanisms against soil-borne root pathogens (Fusarium oxysporum and Sclerotium rolfsii) of the medical plant Aconitum carmichaelii Debx. Methods and results The effects of P. griseofulvum strain CF3 were evaluated with regard to the hyphal growth of S. rolfsii and F. oxysporum, the sclerotial formation and germination of S. rolfsii and its expression of sclerotia-formation-related genes. A field experiment was conducted to explore how strain CF3 controls the severity of soil-borne diseases, promotes the growth of A. carmichaelii plants and mediates shifts in the culturable rhizosphere microbial populations. The results showed that treatment with a cell-free culture filtrate of strain CF3 considerably inhibited the hyphal growth of both S. rolfsii and F. oxysporum, in addition to limiting the sclerotial formation and germination of S. rolfsii. Three genes related to sclerotial formation (ArsclR, ArnsdD1 and ArnsdD2) were predicted in S. rolfsii and their expression was found suppressed by the CF3 treatment. Field application of the CF3 biocontrol agent in a powder form (1·9 × 1010 conidia per gram of substrate) reduced soil-borne disease severity by 15·0%. The shoot and root growth of A. carmichaelii plants was promoted by 61·6 and 83·1% respectively, as the biocontrol strain massively colonized the rhizosphere soil. The CF3 treatment also markedly reduced the density of some known species harmful to plants while increasing the density of some beneficial species in the rhizosphere soil. Significance and impact of the study Genes related to sclerotia formation of S. rolfsii are predicted for the first time and their expression patterns in the presence of P. griseofulvum strain CF3 are evaluated. This comprehensive study provides a candidate fungal biocontrol strain and reveals its potential mechanisms against S. rolfsii and F. oxysporum in A. carmichaelii plants.
19 citations
TL;DR: This work demonstrates the risk posed by CPA on dry-fermented sausages, and provides a successful strategy to prevent this hazard.
18 citations
TL;DR: Four new (penigrisacids A–D, 1–4) and one known (5) carotane sesquiterpenoids were isolated from the deep-sea-derived fungus Penicillium griseofulvum and showed weak cytotoxicity against ECA-109 tumor cells.
Abstract: Four new (penigrisacids A–D, 1–4) and one known (5) carotane sesquiterpenoids were isolated from the deep-sea-derived fungus Penicillium griseofulvum, along with four known compounds (6–9). The planar structures and relative configurations of the new compounds were determined by extensive analysis of the NMR and HRESIMS data. The absolute configurations were established by comparison of the experimental and calculated ECD (electronic circular dichroism) spectra or OR (optical rotation) value. Compound 9 exhibited potent anti-food allergic activity with IC50 value of 28.7 μM, while 4 showed weak cytotoxicity against ECA-109 tumor cells (IC50 = 28.7 μM).
15 citations
TL;DR: A novel azaphilone compound, called chlamyphilone, with significant insecticidal activity, has been isolated from the culture filtrate of P. chlamydosporia, and its structure has been determined using extensive spectroscopic methods and chemical derivatization.
Abstract: Metabolites from a collection of selected fungal isolates have been screened for insecticidal activity against the aphid Acyrthosiphon pisum. Crude organic extracts of culture filtrates from six fungal isolates (Paecilomyces lilacinus, Pochonia chlamydosporia, Penicillium griseofulvum, Beauveria bassiana, Metarhizium anisopliae and Talaromyces pinophilus) caused mortality of aphids within 72 h after treatment. In this work, bioassay-guided fractionation has been used to characterize the main bioactive metabolites accumulated in fungal extracts. Leucinostatins A, B and D represent the bioactive compounds produced by P. lilacinus. From P. griseofulvum and B. bassiana extracts, griseofulvin and beauvericin have been isolated, respectively; 3-O-Methylfunicone and a mixture of destruxins have been found in the active fractions of T. pinophilum and M. anisopliae, respectively. A novel azaphilone compound, we named chlamyphilone, with significant insecticidal activity, has been isolated from the culture filtrate of P. chlamydosporia. Its structure has been determined using extensive spectroscopic methods and chemical derivatization.
11 citations
TL;DR: It is necessary to reduce aw on the surface of the hams below 0.85 during ripening before to increase temperature to reduce growth of P. griseofulvum and CPA production.
Abstract: Cyclopiazonic acid (CPA)-producing Penicillium griseofulvum is usually found on the dry-cured ham surface during its ripening. The objective of this work was to evaluate the effect of temperature and water activity (aw) of dry-cured ham processing on growth, CPA production, and temporal relative expression of genes involved in CPA biosynthesis on dry-cured meat-based media. P. griseofulvum CECT 2919 grew faster than P. griseofulvum IBT 14319 in all conditions tested, although no growth occurred at 0.85 aw. Besides, the dry-cured ham-based medium favoured CPA synthesis for both strains compared to the meat-based medium. For the strain CECT 2919, the expression of the mfs-1 and pks-nrps genes were stimulated at 0.90 and 0.95 aw, respectively, while the dmaT gene expression was inhibited during the incubation time. By contrast, the strain IBT 14319 showed that the dmaT gene expression was stimulated at 0.90 aw, while the pks-nrps and mfs-1 genes were repressed throughout incubation time. In conclusion, it is necessary to reduce aw on the surface of the hams below 0.85 during ripening before to increase temperature to reduce growth of P. griseofulvum and CPA production. This information may be useful to design preventive and corrective actions to minimise risks associated with the presence of CPA in dry-cured ham.
4 citations
Patent•
29 Nov 2019
TL;DR: In this paper, the authors described the preparation and application of Penicillium griseofulvum ZZ380, which is obtained by separation and culture from marine animal pachygrapsus crassipes.
Abstract: The invention provides penicipyrrodiether A, preparation and application thereof. Penicillium griseofulvum is classified and named as Penicillium griseofulvum ZZ380, and is obtained by separation andculture from marine animal pachygrapsus crassipes. According to the invention, penicillium griseofulvum ZZ380 is fermented to obtain penicipyrrodiether A, which has the activity of resisting methicillin-resistant staphylococcus aureus, can remarkably inhibit the growth of drug-resistant bacteria MRSA, has application prospects in treatment of drug-resistant bacteria MRSA infection, and can be applied to preparation of drugs resisting methicillin-resistant staphylococcus aureus. The chemical structural formula of penicipyrrodiether A is shown as the specification.
Patent•
27 Aug 2019
TL;DR: In this article, an anti-glioma active substance pyrrospirone G was obtained from a known strain Penicillium griseofulvum ZZ380 by a specific culture method.
Abstract: The invention provides a preparation method of an anti-glioma active substance pyrrospirone G, which is obtained from a known strain Penicillium griseofulvum ZZ380 by a specific culture method. The method of the invention greatly increases the yield of pyrrospirone G, overcomes the defect in the prior art that the yield in a BMPM liquid medium is very low. The pyrrospirone G has the characteristics of high anti-glioma activity and low toxicity, experiments show that the pyrrospirone G has significant effect of inhibiting the proliferation of human glioma cells, is less toxic to normal human gliocyte and can be combined with pharmaceutically acceptable carriers into the medicines of various dosage forms for the treatment of gliomas. Therefore, pyrrospirone G has broad application prospectsin the preparation of anti-glioma medicines.
Patent•
10 Sep 2019
TL;DR: In this paper, a preparation method of an anti-glioma active substance penicillium alkali ether A was proposed, which utilizes a marine strain of penicllium griseofulvum ZZ380.
Abstract: The invention provides a preparation method of an anti-glioma active substance penicillium alkali ether A, which utilizes a marine strain of penicillium griseofulvum ZZ380, cultures penicillium griseofulvum by utilizing a rice medium, greatly improves the yield of the anti-glioma active substance penicillium alkali ether A, and overcomes shortcomings of low yield of the penicillium alkali ether Aprepared by the prior art. The penicillium alkali ether A has significant inhibitory activity on human glioma cell proliferation, exhibits the characteristic of strong anti-glioma activity of the penicillium alkali ether A, and can be combined with a pharmaceutically acceptable carrier to form various dosage forms of drugs, and is used for treating glioma. Therefore, the penicillium alkali ether Ahas a good application prospect in preparation of anti-glioma drugs.