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Penicillium griseofulvum

About: Penicillium griseofulvum is a research topic. Over the lifetime, 169 publications have been published within this topic receiving 2702 citations.


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01 Jan 2014
TL;DR: This paper deals with the isolation, screening, molecular characterization and phylogenetic analysis of proteolytic fungi, and three promising proteolytics fungi were selected for further investigations.
Abstract: This paper deals with the isolation, screening, molecular characterization and phylogenetic analysis of proteolytic fungi. Forty seven fungal isolates were preliminarily screened for their ability to produce protease on skimmed milk agar. Among the forty seven isolates, thirty four isolates were protease positive. Based on the secondary screening, three promising proteolytic fungi were selected for further investigations. The DNA was extracted from the fungi, ITS-rDNA amplified by PCR and its ITS regions were sequenced. Based on the molecular characterization and phylogenetic studies, the selected isolates were identified as Penicillium citrinum LCJ 222 (Accession no. KF414682), Penicillium griseofulvum LCJ 231 (Accession no. KF414683) and Penicillium citrinum LCJ 233 (Accession no. KF414684).

4 citations

Journal ArticleDOI
TL;DR: Based on differences in internal transcribed spacer (ITS) sequences of Penicillium genus and specific isoamyl alcohol oxidase (IAO) sequences, species-specific primers AS1/RS4 and IAO1/IAO2 were designed and synthesized which were then employed in optimized PCR systems.
Abstract: Molecular methods were carried out to detect Penicillium griseofulvum, a dominant species related to heavy metal pollution, which was screened from marine contaminated sediments. Based on differences in internal transcribed spacer (ITS) sequences of Penicillium genus and specific isoamyl alcohol oxidase (IAO) sequences, species-specific primers AS1/RS4 and IAO1/IAO2 of Penicillium griseofulvum were designed and synthesized which were then employed in optimized PCR systems. The detection sensitivities were compared through ordinary PCR and nested-PCR using two pairs of primers, respectively. Both primer pairs could exclusively amplify destined DNA fragment from contaminated environmental samples in our researches. As for primers AS1/RS4, the detection sensitivity for spores (pure spore DNA) could be 10 fg/μl and 10 spores, respectively, and the detection sensitivity for the sediments was 102 spores/0.25 g sediments. While the detection sensitivity of IAO1/IAO2 primers was lower than that of AS1/RS4. Despite the difference in detection sensitivity, it is feasible that the species-specific primers could be used as probes for the detection of environmental pollution dominant species, Penicillium griseofulvum, since the frequency of occurrence and amount of this strain could preferably indicate the pollution degree.

3 citations

01 Jan 2013
TL;DR: This study showed that all tested Penicillium isolates were virulent for apple fruits and in vitro toxigenic, capable of producing patulin, the characteristic mycotoxin of Penicllium species.
Abstract: Mycotoxin production and pathogenicity of Penicillium spp. involved in apple blue mold were investigated. Twenty four isolates representing nine species recovered from blue moldy apple samples were used. Mycotoxin production of these isolates was assayed using HPLC. Statistical analysis of pathogenicity test was undertaken and LSD was used to compare means. Mycotoxin assay revealed that all isolates were capable of producing patulin in their culture media with the highest production (19.70 ppm) from Penicillium griseofulvum isolate No. 14. In respect of other mycotoxins, Penicillium puberulum isolate No. 17 was the highest producer of citrinin (21.63 ppm), while Penicillium verrucosum isolate No. 22 was the highest producer of penicillic acid (8 ppm). Pathogenicity test revealed that all Penicillium isolates were pathogenic, exhibiting variable disease severity toward infested apple fruits. This study showed that all tested Penicillium isolates were virulent for apple fruits and in vitro toxigenic, capable of producing patulin, the characteristic mycotoxin of Penicillium species.

3 citations

Journal ArticleDOI
TL;DR: The introduction of suitable antagonistic microorganisms into the rhizosphere could decrease the number of phytotoxic micromycetes and improve the growth of apple seedlings.

3 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20214
20207
20198
201811
20176
20167