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Penicillium griseofulvum

About: Penicillium griseofulvum is a research topic. Over the lifetime, 169 publications have been published within this topic receiving 2702 citations.


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Journal ArticleDOI
TL;DR: Investigation of the rate and pattern of hydrolysis of PgXynA and PfXynC on wheat soluble arabinoxylan showed the predominant production of xylotriose and xylobiose as end products, suggesting that the specificity region of both Penicillium xylanases spans about six xylose units.
Abstract: Two genes encoding family 11 endo-(1,4)-β-xylanases from Penicillium griseofulvum (PgXynA) and Penicillium funiculosum (PfXynC) were heterologously expressed in Escherichia coli as glutathione S-transferase fusion proteins, and the recombinant enzymes were purified after affinity chromatography and proteolysis. PgXynA and PfXynC were identical to their native counterparts in terms of molecular mass, pI, N-terminal sequence, optimum pH, and enzymatic activity towards arabinoxylan. Further investigation of the rate and pattern of hydrolysis of PgXynA and PfXynC on wheat soluble arabinoxylan showed the predominant production of xylotriose and xylobiose as end products. The initial rate data from the hydrolysis of short xylo-oligosaccharides indicated that the catalytic efficiency increased with increasing chain length (n) of oligomer up to n = 6, suggesting that the specificity region of both Penicillium xylanases spans about six xylose units. In contrast to PfXynC, PgXynA was found insensitive to the wheat xylanase inhibitor protein XIP-I.

57 citations

Journal ArticleDOI
TL;DR: Evaluating different species of Penicillium to identify those which have the potential to produce the greatest amount of the mycotoxin, patulin found that potato dextrose broth supplemented with manganese was optimal for maximum production of patulin.

56 citations

Journal ArticleDOI
TL;DR: The investigation demonstrated that Ps1-1 was a galactofuranose-containing galactomannan differing from previously described extracellular polysaccharides, as well as two-dimensional nuclear magnetic resonance (1D and 2D NMR) and mass spectroscopic analyses.

53 citations

Journal ArticleDOI
TL;DR: It is suggested that a novel photosensitization mediated curcumin treatment is effective against fungal spores/cells and the variation of percentage reduction was dependent onCurcumin concentration, light dosage and fungal species.
Abstract: The global concerns regarding the emergence of fungicide-resistant strains and the impact of the excessive use of fungicidal practises on our health, food, and environment have increased, leading to a demand for alternative clean green technologies as treatments. Photosensitization is a treatment that utilises a photosensitiser, light and oxygen to cause cell damage to microorganisms. The effect of photosensitization mediated by curcumin on Aspergillus niger, Aspergillus flavus, Penicillium griseofulvum, Penicillium chrysogenum, Fusarium oxysporum, Candida albicans and Zygosaccharomyces bailii was investigated using three methods. The viability of spores/cells suspended in aqueous buffer using different concentrations of curcumin solution (100-1000μM) and light dose (0, 24, 48, 72 and 96J/cm2) were determined. Spraying curcumin solution on inoculated surfaces of agar plates followed by irradiation and soaking spores/cells in curcumin solution prior to irradiation was also investigated. In aqueous mixtures, photosensitised spores/cells of F. oxysporum and C. albicans were inhibited at all light doses and curcumin concentrations, while inactivation of A. niger, A. flavus P. griseofulvum, P. chrysogenum and Z. bailii were highly significant (P<0.001) reduced by 99%, 88.9%, 78%, 99.7% and 99.2% respectively. On the surface of agar plates, spores/cells exposed to a light dose of 360J/cm2 sprayed with curcumin at 800μM showed complete inhibition for A. niger, F. oxysporum, C. albicans and Z. bailii, while A. flavus P. griseofulvum, and P. chrysogenum reduced by 75%, 80.4% and 88.5% respectively. Soaking spores/cells with curcumin solution prior to irradiation did not have a significant effect on the percentage reduction. These observations suggest that a novel photosensitization mediated curcumin treatment is effective against fungal spores/cells and the variation of percentage reduction was dependent on curcumin concentration, light dosage and fungal species.

51 citations

Journal ArticleDOI
TL;DR: Evaluated strains of B. nivea and B. fulva don't seem to be responsible for the occurrence of patulin by lack of genes, but the absence of 6-methylsalicylic acid and isoepoxydon dehydrogenase genes can explain their inability to produce patulin.

49 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20214
20207
20198
201811
20176
20167