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Peptide sequence

About: Peptide sequence is a(n) research topic. Over the lifetime, 84156 publication(s) have been published within this topic receiving 4357443 citation(s).


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01 Jan 1965

6,834 citations

Journal ArticleDOI
Abstract: Quantitative study of protein-protein and protein-ligand interactions in solution requires accurate determination of protein concentration. Often, for proteins available only in "molecular biological" amounts, it is difficult or impossible to make an accurate experimental measurement of the molar extinction coefficient of the protein. Yet without a reliable value of this parameter, one cannot determine protein concentrations by the usual uv spectroscopic means. Fortunately, knowledge of amino acid residue sequence and promoter molecular weight (and thus also of amino acid composition) is generally available through the DNA sequence, which is usually accurately known for most such proteins. In this paper we present a method for calculating accurate (to +/- 5% in most cases) molar extinction coefficients for proteins at 280 nm, simply from knowledge of the amino acid composition. The method is calibrated against 18 "normal" globular proteins whose molar extinction coefficients are accurately known, and the assumptions underlying the method, as well as its limitations, are discussed.

4,933 citations

Journal ArticleDOI
TL;DR: A purified protein derived from the twisted beta-pleated sheet fibrils in cerebrovascular amyloidosis associated with Alzheimer's disease has been isolated and Amino acid sequence analysis and a computer search reveals this protein to have no homology with any protein sequenced thus far.
Abstract: A purified protein derived from the twisted beta-pleated sheet fibrils in cerebrovascular amyloidosis associated with Alzheimer's disease has been isolated by Sephadex G-100 column chromatography with 5 M guanidine-HC1 in 1 N acetic acid and by high performance liquid chromatography. Amino acid sequence analysis and a computer search reveals this protein to have no homology with any protein sequenced thus far. This protein may be derived from a unique serum precursor which may provide a diagnostic test for Alzheimer's disease and a means to understand its pathogenesis.

4,557 citations

Journal ArticleDOI
TL;DR: A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described.
Abstract: A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described. The predictive accuracy is estimated to be around 75-80% for both prokaryotic and eukaryotic proteins.

4,504 citations

Journal ArticleDOI
27 Feb 1987-Cell
TL;DR: This brief review of sequence data from embryogenesis, thrombosis, and lymphocyte help and killing is summarized and attempts to clarify the relationships among the members of this family of cell surface receptors.
Abstract: What do cell adhesion, cell migration during embryogenesis, thrombosis, and lymphocyte help and killing have in common? It has recently become clear that these ostensibly unrelated processes all involve related cell surface receptors. This realization comes from sequence data, which show that cell surface receptors for extracellular matrix proteins, a major glycoprotein complex on platelet cell surfaces, and two groups of glycoprotein antigens on lymphoid and myeloid cells all contain homologous subunits. In this brief review, I summarize data from these diverse fields and attempt to clarify the relationships among the members of this family of cell surface receptors.

4,172 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20227
2021496
2020976
20191,101
20181,193
20171,346