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Showing papers on "Phosphotungstic acid published in 1972"


Journal ArticleDOI
TL;DR: A mixture consisting of 1% phosphotungstic acid in 10% chromic acid selectively stains the plasma membrane of plant cells, retained in homogenates and serves to identify the plant plasma membrane in cell fractions.
Abstract: A mixture consisting of 1% phosphotungstic acid (PTA) in 10% chromic acid (CrO3) selectively stains the plasma membrane of plant cells. Whole tissue or pelleted cell fractions are prepared for electron microscopy using conventional methods including glutaraldehyde fixation and OsO4 postfixation, dehydration in acetone and embedding in Epon. To stain the plasma membrane, thin sections are transferred with a plastic loop to the surface of a 1% aqueous solution of periodic acid for 30 min for destaining. Following transfer through 5 distilled water rinses, the sections are exposed to the PTA-CrO3 mixture for 5 min, rinsed and mounted on grids for viewing with the electron microscope. The selectivity of the stain is retained in homogenates and serves to identify the plant plasma membrane in cell fractions.

247 citations


Journal ArticleDOI
TL;DR: A rapid, convenient one-tube method for serum uric acid is described which employs a single addition of phospho-18-tungstic acid, which yields results in excellent agreement with the phosphotungstate-uric acid method of Henry et al.

55 citations





Journal ArticleDOI
TL;DR: A new method based on the colour reaction of uric acid with phosphotungstic acid in an alkaline solution, using the Auto Analyzersystem, is reported, which can be useful as a routine test for uric Acid in clinical laboratories.
Abstract: A new method for the automated determination of uric acid in serum and urine, using the Auto Analyzersystem, is reported. This method is based on the colour reaction of uric acid with phosphotungstic acid in an alkaline solution. The results obtained by this method are satisfactory and reliable. It is considered that the method can be useful as a routine test for uric acid in clinical laboratories.