Showing papers on "Phosphotungstic acid published in 1975"
TL;DR: In this paper, a post-mitochondrial supernanant from sugarcane cells grown in suspension culture was separated by sucrose density centrifugation and examined for presence of plasma membrane.
25 citations
TL;DR: An automated colorimetric method for determination of uric acid in serum or urine by use of an AutoAnalyzer II or SMA 12/60 (Technicon Corp.) is described, which shows good correlation with results of the Technicon phosphotungstic acid reduction method.
Abstract: We describe an automated colorimetric method for determination of uric acid in serum or urine by use of an AutoAnalyzer II or SMA 12/60 (Technicon Corp.). The method depends on reduction of a ferric-phenanthroline complex by uric acid under acidic conditions to a ferrous-phenanthroline complex, which absorbs at 505 nm. Advantages of this method over other methods now in use are that color and concentration are linearly related (to 20 mg/100 ml); aqueous reagents are easily prepared, stable, and inexpensive; and interference from ascorbic acid has been eliminated by use of an alkaline copper-containing diluent. The analysis is also free of interference from glucose, creatinine, glutathione, salicylates and hemoglobin. Correlation with results of the Technicon phosphotungstic acid reduction method is excellent ( r = .996). Correlation with results of an automated uricase method is satisfactory ( r = .979). Recovery of uric acid was 101% over a wide concentration range.
11 citations
TL;DR: In this article, the authors investigated the difference in catalytic activity between phosphotungstic acid-alumina catalysts for propyleneethylene codimerization by calcining the catalysts at temperatures from 300 to 800 °C.
11 citations
TL;DR: Measurements of the parameters of the dense projections suggest that the configuration of the Presynaptic vesicular grid is not uniform along the presynaptic areas.
Abstract: Studies have been carried out on the synapses in the cerebral cortex of rat by using impregnation with ethanolic solution of phosphotungstic acid, contrast staining with ruthenium red and impregnation with bismuth iodide, with or without subsequent uranyl acetate and lead citrate staining. It has been established that dense projections are adequately visualized with methods demonstrating basic chemical groups (phosphotungstic acid and bismuth iodide), whereas the synaptic vesicles are stained by techniques demonstrating acid chemical groups (ruthenium red and uranyl acetate and lead citrate). On the basis of these observations a hypothesis is forwarded concerning the mechanisms of migration of synaptic vesicles towards the presynaptic membrane. Measurements of the parameters of the dense projections suggest that the configuration of the presynaptic vesicular grid is not uniform along the presynaptic areas.
9 citations
Journal Article•
8 citations
Patent•
11 Sep 1975
TL;DR: A container for a solution containing heteropolyacid ions, at least that surface of the container which makes contact with the solution containing HPI, is defined in this article, and the container is made of an alloy comprising 2 to 7% of nickel, 19 to 27% by weight of chromium, not more than 0.5% of carbon and the remainder being iron and minor elements.
Abstract: A container for a solution containing heteropolyacid ions, at least that surface of the container which makes contact with the solution containing heteropolyacid ions selected from the group consisting of silicotungstic acid, borotungstic acid, phosphotungstic acid, silicomolybdic acid and phosphomolybdic acid and being made of an alloy comprising 2 to 7% by weight of nickel, 19 to 27% by weight of chromium, not more than 0.5% by weight of carbon and the remainder being iron and minor elements.
6 citations