Showing papers on "Photoacoustic spectroscopy published in 2019"

Atmospheric CH4 measurement near a landfill using an ICL-based QEPAS sensor with V-T relaxation self-calibration

Abstract: A quartz-enhanced photoacoustic spectroscopy methane (CH4) sensor with vibrational to translational (V-T) relaxation self-calibration was realized and tested for atmospheric CH4 detection near a landfill. To normalize the influence of H2O vapor on the CH4 energy relaxation rate, CH4 and H2O concentrations were detected simultaneously by means of a frequency division multiplexing technique, in which a custom quartz tuning fork was operated in the fundamental and first overtone combined vibration mode. A continuous wave, thermoelectrically cooled distributed feedback interband cascade laser emitting at 3.3 μm and a near-infrared DFB laser emitting at 1.37 μm were used as the excitation source for CH4 and H2O detection, respectively. A theoretical model of V-T relaxation and self-calibration method were developed to allow this CH4 sensor to have a simple setup and a small sensor size. Continuous field measurements were carried out near the largest sanitary landfill in Shanxi province, China, to demonstrate the stability and ruggedness of the realized CH4 sensor.

Ultra-high sensitive light-induced thermoelastic spectroscopy sensor with a high Q-factor quartz tuning fork and a multipass cell.

Abstract: An ultra-high sensitive light-induced thermoelastic spectroscopy (LITES) sensor based on a resonant high Q-factor quartz turning fork (QTF) and a Herriot multipass cell was demonstrated for the first time, to the best of our knowledge. The performance of LITES and widely used tunable diode laser absorption spectroscopy (TDLAS) were experimentally investigated and compared at the same conditions. Carbon monoxide (CO) was chosen as the analyte to verify the reported sensors’ performance. With a minimum detection limit (MDL) of 470 ppb for 60 ms integration time and a noise equivalent absorption (NEA) coefficient of 2.0×10−7 cm−1 Hz−1/2, and a MDL of 17 ppb with an optimum integration time of 800 s, the reported LITES sensor showed a superior sensing capability compared with a TDLAS sensor and a conventional quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor.

Tuning forks with optimized geometries for quartz-enhanced photoacoustic spectroscopy

Abstract: We report on the design, realization, and performance of novel quartz tuning forks (QTFs) optimized for quartz-enhanced photoacoustic spectroscopy (QEPAS). Starting from a QTF geometry designed to provide a fundamental flexural in-plane vibrational mode resonance frequency of ~16 kHz, with a quality factor of 15,000 at atmospheric pressure, two novel geometries have been realized: a QTF with T-shaped prongs and a QTF with prongs having rectangular grooves carved on both surface sides. The QTF with grooves showed the lowest electrical resistance, while the T-shaped prongs QTF provided the best photoacoustic response in terms of signal-to-noise ratio (SNR). When acoustically coupled with a pair of micro-resonator tubes, the T-shaped QTF provides a SNR enhancement of a factor of 60 with respect to the bare QTF, which represents a record value for mid-infrared QEPAS sensing.

Ppb-Level Quartz-Enhanced Photoacoustic Detection of Carbon Monoxide Exploiting a Surface Grooved Tuning Fork.

Abstract: A compact and sensitive carbon monoxide (CO) sensor was demonstrated by using quartz enhanced photoacoustic spectroscopy (QEPAS) exploiting a novel 15.2 kHz quartz tuning fork (QTF) with grooved surfaces. The custom QTF was designed to provide a quality factor as high as 15 000 at atmospheric pressure, which offers a high detection sensitivity. A large QTF prong spacing of 800 μm was selected, allowing one to avoid the use of any spatial filters when employing a quantum cascade laser as the excitation source. Four rectangular grooves were carved on two prong surfaces of the QTF to decrease the electrical resistance and hence enhance the signal amplitude. With water vapor as the catalyst for vibrational energy transfer, the sensor system using the novel surface grooved QTF achieved a CO minimum detection limit of 7 ppb for a 300 ms averaging time, which corresponds to a normalized noise equivalent absorption coefficient of 8.74 × 10–9 cm–1W /√Hz. Continuous measurements covering a seven-day period for atmo...

Highly sensitive acetylene detection based on multi-pass retro-reflection-cavity-enhanced photoacoustic spectroscopy and a fiber amplified diode laser

Abstract: In this paper, a multi-pass retro-reflection-cavity-enhanced photoacoustic spectroscopy (PAS) based gas sensor is reported for the first time. The multi-pass retro-reflection-cavity consisted of two right-angle prisms and was designed to reflect the laser beam to pass through the photoacoustic (PA) cell four times, which improved the acetylene (C2H2)-PAS sensor signal level significantly. The optical power of a near-infrared distributed feedback (DFB) diode laser emitting a continuous wave (CW) was amplified to 1000 mW with an erbium-doped fiber amplifier. The background noise was reduced with wavelength modulation spectroscopy (WMS) and 2nd harmonic demodulation techniques. The linear optical power and concentration response of such a PAS sensor were investigated, and the experimental results showed excellent characteristics. When the integration the time of the sensor system was set to 1 s, the minimum detection limit (MDL) for C2H2 detection was 8.17 ppb, which corresponds to a normalized noise equivalent absorption coefficient (NNEA) of 1.84 × 10-8 cm-1W/√Hz. The long-term stability of such a multi-pass retro-reflection-cavity-enhanced PAS based C2H2 sensor was evaluated by an Allan deviation analysis. It was demonstrated that the multi-pass retro-reflection-cavity-enhanced PAS sensor had an excellent stability. An MDL of 600 ppt was achieved when the integration time was set to ~1000 s. It was verified that the method of multi-pass retro-reflection-cavity-enhanced PAS with an amplified laser source improved the sensor performance significantly. If an appropriate cavity design with increasing reflection times is used, the MDL of such a PAS-based sensor can be further improved.

Parts-per-billion-level detection of hydrogen sulfide based on near-infrared all-optical photoacoustic spectroscopy

Abstract: A highly sensitive trace hydrogen sulfide (H2S) gas sensing scheme based on all-optical photoacoustic spectroscopy is demonstrated. A high-power erbium-doped fiber amplified near-infrared laser is used as a light source for acoustic excitation. Meanwhile, the second-harmonic photoacoustic signal is measured by a fiber-optic cantilever microphone which is equipped with a white-light interferometric readout. For sensitivity improvement, the demodulated digital photoacoustic signal is processed by a virtual lock-in amplifier. The continuous H2S measurement experiment shows the ability of real-time response. A detection limit is achieved to be 33 ppb with a 10 s measurement time at the wavelength of 1576.29 nm. With both the excitation light and the probe light being transmitted by optical fibers, the designed sensing system has the advantages of remote detection and immunity to electromagnetic interference.

Acoustic Coupling between Resonator Tubes in Quartz-Enhanced Photoacoustic Spectrophones Employing a Large Prong Spacing Tuning Fork.

Abstract: A theoretical model describing the acoustic coupling between two resonator tubes in spectrophones exploiting custom-made quartz tuning forks (QTFs) is proposed. The model is based on an open-end correction to predict the optimal tube length. A calculation of the sound field distribution from one tube exit allowed for the estimation of the optimal radius as a function of the QTF prong spacing and the sound wavelength. The theoretical predictions have been confirmed using experimental studies employing a custom QTF with a fundamental flexural mode resonance frequency of 15.8 kHz and a quality factor of 15,000 at atmospheric pressure. The spacing between the two prongs was 1.5 mm. Spectrophones mounting this QTF were implemented for the quartz-enhanced photoacoustic detection of water vapor in air in the mid-infrared spectral range.

Compact and sensitive mid-infrared all-fiber quartz-enhanced photoacoustic spectroscopy sensor for carbon monoxide detection.

Abstract: A compact and sensitive quartz-enhanced photoacoustic spectroscopy (QEPAS) based sensor for carbon monoxide (CO) detection was demonstrated by using a mid-infrared all-fiber structure as well as a 3D-printed acoustic detection module. An all-fiber configuration has advantages of easier optical alignment, lower insertion loss, improvement in system stability, reduction in sensor size and lower cost. The 3D-printed acoustic detection module was introduced to match the mid-infrared all-fiber structure and further decrease the sensor volume, which resulted in a small size of 3.5 cm3 and a weight of 5 grams. A 2.33 μm distributed feedback fiber-coupled diode laser was used as the laser excitation source. A custom quartz tuning fork (QTF) with a small-gap of 200 μm was used as the acoustic wave transducer in order to improve the signal level of the QEPAS sensor. An acoustic micro resonator was utilized as the acoustic wave enhancer. The gas pressure and laser wavelength modulation depth were optimized, respectively. Water vapor was used to accelerate the vibrational-translational relaxation rate of the targeted CO molecule. Finally, a minimum detection limit (MDL) of 4.2 part per million (ppm) was achieved, corresponding to a normalized noise equivalent absorption (NNEA) coefficient of 7.4 × 10−9 cm−1W/√Hz. An Allan deviation analysis was used to evaluate the long-term stability of the reported CO-QEPAS sensor system. With an integration time of 150 s, the MDL was improved to be 1.3 ppm.

Ultrasensitive photoacoustic detection in a high-finesse cavity with Pound-Drever-Hall locking.

Abstract: We demonstrate an ultrasensitive photoacoustic sensor using a low laser power (4 mW) and high-finesse (>9000) optical cavity. The Pound–Drever–Hall (PDH) method is adopted to lock the external cavity diode laser at 1531.58 nm to the Fabry–Perot cavity. By placing a photoacoustic cell inside the 130-mm-long optical cavity, we obtain an enhancement of more than 630 times in laser power for acetylene (C2H2) detection. The present photoacoustic spectroscopy (PAS) sensor achieves a normalized noise equivalent absorption coefficient of 1.1×10−11 cm−1 WHz−1/2, which is unprecedented sensitivity among all the current PAS sensors. Our results demonstrate the feasibility of merging PAS with a high-finesse cavity using PDH locking for ultrasensitive trace gas detection.

Off-beam QEPAS sensor using an 11-μm DFB-QCL with an optimized acoustic resonator

Abstract: An off-beam quartz-enhanced photoacoustic spectroscopy (QEPAS) sensor was designed for ethylene detection using a distributed-feedback quantum cascade laser (QCL) operating in the mid-infrared around 11 μm. The acoustic microresonator configuration was experimentally optimized using an original open-cell photoacoustic setup with a MEMS microphone. Correction factors based on theoretical acoustic models were introduced in order to accurately describe the response of millimeter-sized acoustic resonators. The optimized QEPAS sensor exhibited a limit of detection of 60 ppb for 60 s integration, giving a NNEA of 4.8 × 10−8 W·cm−1·Hz-0.5.

Photoacoustic imaging of cancer cells with glycol-chitosan-coated gold nanoparticles as contrast agents.

Abstract: Utility of glycol-chitosan-coated gold nanoparticles (GC-AuNPs) as a photoacoustic contrast agent for cancer cell imaging was demonstrated. Through the synergistic effect of glycol chitosan and gold nanoparticles, GC-AuNPs showed cellular uptake in breast cancer cells and resulted in strong photoacoustic signals in tissue-mimicking cell phantoms. The performance of GC-AuNPs as contrast agents was established with photoacoustic imaging and confirmed with dark-field microscopy. The cell phantoms displayed strong photoacoustic signals if cells were incubated more than 3 h with GC-AuNPs, compared with PEG-AuNPs that showed no photoacoustic signal increase. The enhanced photoacoustic signals originated from the plasmon coupling effect of GC-AuNPs after the cellular uptake in cancer cells. Importantly, photoacoustic imaging of cancer cells was achieved with GC-AuNPs—contrast agents that did not require antibodies or complex surface modification. The endocytosis of GC-AuNPs was also confirmed with dark-field microscopy. The results show that GC-AuNPs have potential as a photoacoustic contrast agent for cellular imaging including tumor tissue imaging.

Development of a blood oxygenation phantom for photoacoustic tomography combined with online pO2 detection and flow spectrometry.

Abstract: Photoacoustic tomography (PAT) is intrinsically sensitive to blood oxygen saturation (sO2) in vivo. However, making accurate sO2 measurements without knowledge of tissue- and instrumentation-related correction factors is extremely challenging. We have developed a low-cost flow phantom to facilitate validation of PAT systems. The phantom is composed of a flow circuit of tubing partially embedded within a tissue-mimicking material, with independent sensors providing online monitoring of the optical absorption spectrum and partial pressure of oxygen in the tube. We first test the flow phantom using two small molecule dyes that are frequently used for photoacoustic imaging: methylene blue and indocyanine green. We then demonstrate the potential of the phantom for evaluating sO2 using chemical oxygenation and deoxygenation of blood in the circuit. Using this dynamic assessment of the photoacoustic sO2 measurement in phantoms in relation to a ground truth, we explore the influence of multispectral processing and spectral coloring on accurate assessment of sO2. Future studies could exploit this low-cost dynamic flow phantom to validate fluence correction algorithms and explore additional blood parameters such as pH and also absorptive and other properties of different fluids.

Broadband photoacoustic spectroscopy of CH414 with a high-power mid-infrared optical frequency comb.

Abstract: We report a photoacoustic spectroscopy setup with a high-power mid-infrared frequency comb as the light source. The setup is used in broadband spectroscopy of radiocarbon methane. Owing to the high sensitivity of a cantilever-enhanced photoacoustic cell and the high-power light source, we can reach a detection limit below 100 ppb in a broadband measurement with a sample volume of only a few milliliters. The first infrared spectrum of CH414 is reported and given a preliminary assignment. The results lay a foundation for the development of optical detection systems for radiocarbon methane.

High-resolution terahertz spectroscopy with quantum-cascade lasers

Abstract: Terahertz (THz) quantum-cascade lasers (QCLs) are narrow band, high-power, and frequency-agile sources. These properties make them attractive for applications in high-resolution molecular and atomic spectroscopy. In the past few years, various techniques and methods regarding high-resolution spectroscopy with THz QCLs have been developed, namely, direct absorption spectroscopy, detection schemes such as wavelength and frequency modulation, differential spectroscopy, photoacoustic spectroscopy, and heterodyne spectroscopy. We briefly review the most relevant technological and methodological advances in this field, and we discuss perspectives and future directions.

Damping Mechanisms of Piezoelectric Quartz Tuning Forks Employed in Photoacoustic Spectroscopy for Trace Gas Sensing

Abstract: A study of the dependence of main loss mechanisms on the geometry of piezoelectric quartz tuning forks (QTFs) is reported. The influence of these loss mechanisms on the quality factor Q occurring while the QTF vibrates at the in-plane flexural fundamental and first overtone resonance modes is investigated. From this study, two QTFs efficiently operating both at the fundamental and first overtone mode are designed and realized. Data analysis demonstrates that air viscous damping is the dominant energy dissipation mechanism for both flexural modes. However, at the first overtone mode the air damping is reduced and higher quality factors can be obtained when operating at the first overtone mode with respect to the fundamental one.

A portable gas sensor for sensitive CO detection based on quartz-enhanced photoacoustic spectroscopy

Abstract: A compact, portable and rechargeable sensor platform based on quartz-enhanced photoacoustic spectroscopy (QEPAS) was demonstrated for the sensitive detection of carbon monoxide (CO). The acoustic detection module and the circuit components were both integrated which formed a sensor system with a weight of 3.2 kg and dimensions of 250 × 250 × 50 mm3. Water vapor was added to the gas mixture in order to accelerate the vibration translation rate of CO molecules and improve the CO-QEPAS signal performance. An Allan deviation analysis was performed to assess the long-term performance of such a sensor system. For an integration time of 800 s, a minimum detection limit (MDL) of 21 ppb was obtained. To demonstrate the practical application of the reported compact and portable QEPAS sensor, a continuous measurement of CO from the combustion of three kinds of burning materials was performed.

A fiber-tip photoacoustic sensor for in situ trace gas detection.

Abstract: Most trace gas detection methods developed so far largely rely on active sampling procedures, which are known to introduce different kinds of artifacts. Here, we demonstrate sampling-free in situ trace gas detection in millimeter scale volumes with fiber coupled cantilever enhanced photoacoustic spectroscopy. Our 2.4 mm diameter fiber-tip sensor is free from the wavelength modulation induced background signal (a phenomenon that is often overlooked in photoacoustic spectroscopy) and reaches a normalized noise equivalent absorption coefficient of 1.3 × 10-9 W cm-1 Hz-1/2 for acetylene detection. To validate its in situ gas detection capability, we inserted the sensor into a mini fermenter for headspace monitoring of CO2 production during yeast fermentation. Our results show that the sensor can easily follow the different stages of the CO2 production of the fermentation process in great detail.

Quartz tuning fork embedded off-beam quartz-enhanced photoacoustic spectroscopy

Abstract: In order to achieve a high acoustic coupling strength and detection sensitivity and to simplify the assembly and alignment process in quartz-enhanced photoacoustic spectroscopy (QEPAS) technique, a novel quartz tuning fork (QTF) embedded off-beam QEPAS (E-OB-QEPAS) spectrophone was proposed. The structural parameters of the acoustic micro-resonator of the E-OB-QEPAS spectrophone were optimized for enhancing the signal-to-noise ratio gain based on experimental investigation. Compared with the on-beam configuration using a bare QTF, a detection sensitivity enhancement by a factor of ∼25 was achieved by embedding the QTF in one resonant tube. By using two resonant tubes simultaneously embedded with a QTF, dual-channel detection and a two-fold photoacoustic signal enhancement were realized and a detection sensitivity enhancement by a factor of ∼20 and ∼40 were achieved for the single-tube-enhanced and dual-tube-enhanced E-OB-QEPAS spectrophone, respectively.

Single-pixel camera photoacoustic tomography.

Abstract: Since it was first demonstrated more than a decade ago, the single-pixel camera concept has been used in numerous applications in which it is necessary or advantageous to reduce the channel count, cost, or data volume. Here, three-dimensional (3-D), compressed-sensing photoacoustic tomography (PAT) is demonstrated experimentally using a single-pixel camera. A large area collimated laser beam is reflected from a planar Fabry–Perot ultrasound sensor onto a digital micromirror device, which patterns the light using a scrambled Hadamard basis before it is collected into a single photodetector. In this way, inner products of the Hadamard patterns and the distribution of thickness changes of the FP sensor—induced by the photoacoustic waves—are recorded. The initial distribution of acoustic pressure giving rise to those photoacoustic waves is recovered directly from the measured signals using an accelerated proximal gradient-type algorithm to solve a model-based minimization with total variation regularization. Using this approach, it is shown that 3-D PAT of imaging phantoms can be obtained with compression rates as low as 10%. Compressed sensing approaches to photoacoustic imaging, such as this, have the potential to reduce the data acquisition time as well as the volume of data it is necessary to acquire, both of which are becoming increasingly important in the drive for faster imaging systems giving higher resolution images with larger fields of view.

Broadband photoacoustic spectroscopy of $^{14}\text{CH}_4$ with a high-power mid-infrared optical frequency comb

Abstract: We report a photoacoustic spectroscopy setup with a high-power mid-infrared frequency comb as the light source. The setup is used in broadband spectroscopy of radiocarbon methane. Due to the high sensitivity of a cantilever-enhanced photoacoustic cell and the high power light source, we can reach a detection limit below 100 ppb in a broadband measurement with a sample volume of only a few milliliters. The first infrared spectrum of $^{14}\text{CH}_4$ is reported and given a preliminary assignment. The results lay a foundation for the development of optical detection systems for radiocarbon methane.

Assessing relative humidity dependent photoacoustics to retrieve mass accommodation coefficients of single optically trapped aerosol particles

Abstract: Photoacoustic spectroscopy is widely used to measure the light absorption of aerosols. However, the impact of key factors such as the effect of relative humidity and mass exchange on photoacoustic measurements are still poorly understood. We assess such measurement biases and their physical origin by analysing the photoacoustic signal of single tetraethylene glycol (TEG) particles at varying relative humidities. Our results show a decrease in the photoacoustic signal at elevated relative humidities for small particles (0.8–1.5 μm), while for larger sizes (2.2–3.2 μm) the trend is reversed. We model the photoacoustic signal to interpret the observed behaviour in terms of mass and heat flux contribution. The single particle photoacoustic signal analysis presented in this paper additionally allows for the retrieval of the mass accommodation coefficient. Fitting our experimental data to the theoretical model reveals values of αM ≈ 0.02–0.005 for water on TEG in the temperature range 295–309 K.

Sub-ppm-Level Ammonia Detection Using Photoacoustic Spectroscopy with an Optical Microphone Based on a Phase Interferometer

Abstract: A sensitive optical microphone for photoacoustic spectroscopy based on the common path topology of a fibre laser Doppler vibrometer (FLDV) using phase-generated carrier demodulation and a slim diaphragm as an acoustic wave transducer was demonstrated. A resonant gas cell was adapted to enhance gas-detection performance and simultaneously provide efficient cancellation of the window background acoustic signal. Ammonia (NH3) was selected as the target gas. The absorption line was experimentally identified using a distributed feedback laser diode emitting at 1530 nm. The linearity and sensitivity of the gas sensor were measured using wavelength modulation spectroscopy with second harmonic detection. A Teflon diaphragm was used to implement the optical microphone, along with the FLDV, showing a minimum detectable pressure of 79.5 μPa/Hz1/2. The noise-equivalent absorption sensitivity for NH3 detection at the absorption line at 1531.7 nm was 1.85 × 10−8 W cm−1 Hz−1/2, and the limit of detection was 785 ppbv.

A Sensitive Carbon Dioxide Sensor Based on Photoacoustic Spectroscopy with a Fixed Wavelength Quantum Cascade Laser.

Abstract: A photoacoustic spectroscopy (PAS) based carbon dioxide (CO2) sensor with a fixed wavelength quantum cascade laser (FW-QCL) was demonstrated. The emission wavelength of the FW-QCL at 4.42 μm in the mid-infrared spectral region matched a fundamental CO2 absorption line. Amplitude modulation of the laser intensity was used to match the resonant photoacoustic (PA) cell. The noise from the background was reduced with the correlation demodulation technique. The experimental results showed that the sensor had excellent signal stability and a concentration linear response. When the integration time was 1 s, a 1σ minimum detection limit (MDL) of 2.84 parts per million (ppm) for CO2 detection was achieved. The long-term stability of the sensor was evaluated by means of an Allan deviation analysis. With an integration time of ~100 s, the MDL was improved to 1 ppm. This sensor was also used to measure the CO2 concentration from some common emission sources, such as cigarette smoking, automobile exhaust, and the combustion of some carbon-containing materials, which confirmed the stability and robustness of the reported FW-QCL based CO2-PAS sensor system.

Direct and indirect optical transitions in bulk and atomically thin MoS2 studied by photoreflectance and photoacoustic spectroscopy

Abstract: Optical transitions in atomically thin MoS2 samples made by sulfidation of a metallic molybdenum layer have been studied by photoreflectance (PR) and photoacoustic (PA) spectroscopy. The obtained spectra are compared with PR and PA spectra of bulk MoS2. It is shown that the absorption edge observed in the PA spectrum shifts to blue when moving from the bulk MoS2 to the atomically thin MoS2 layers, whereas the direct optical transitions at the K point of the Brillouin zone (A and B transitions), which are observed in the PR spectrum, do not shift spectrally in a significant manner. On the other hand, the AH transition, which is related to the direct optical transition at the H point of the Brillouin zone and is typical of bulk MoS2, is not observed for atomically thin MoS2 layers. Moreover, a strong and broad PR resonance related to the band nesting (C transition) is identified in the PR spectra of studied samples. In this case, C and CH transitions are observed for bulk MoS2, while only a C transition is observed for atomically thin MoS2.

Mid-infrared sensing of CO at saturated absorption conditions using intracavity quartz-enhanced photoacoustic spectroscopy.

Abstract: The sensitivity of quartz-enhanced photoacoustic spectroscopy (QEPAS) can be drastically increased using the power enhancement in high-finesse cavities. Here, low noise resonant power enhancement to 6.3 W was achieved in a linear Brewster window cavity by exploiting optical feedback locking of a quantum cascade laser. The high intracavity intensity of up to 73 W mm−2 in between the prongs of a custom tuning fork resulted in strong optical saturation of CO at 4.59 µm. Saturated absorption is discussed theoretically and experimentally for photoacoustic measurements in general and intracavity QEPAS (I-QEPAS) in particular. The saturation intensity of CO’s R9 transition was retrieved from power-dependent I-QEPAS signals. This allowed for sensing CO independently from varying degrees of saturation caused by absorption induced changes of intracavity power. Figures of merit of the I-QEPAS setup for sensing of CO and H2O are compared to standard wavelength modulation QEPAS without cavity enhancement. For H2O, the sensitivity was increased by a factor of 230, practically identical to the power enhancement, while the sensitivity gain for CO detection was limited to 57 by optical saturation.

A Sensitive Carbon Monoxide Sensor Based on Photoacoustic Spectroscopy with a 2.3 μm Mid-Infrared High-Power Laser and Enhanced Gas Absorption.

Abstract: A photoacoustic spectroscopy (PAS)-based carbon monoxide (CO) gas sensor with a high-power laser and an enhanced gas absorption was demonstrated. The light source was a distributed feedback (DFB), continuous wave (CW) diode laser with a high output power of ~8 mW to give a strong excitation. The target gas received optical absorption enhanced two times by using a right-angle prism reflecting the laser beam. In order to reduce the noise from the background, wavelength modulation spectroscopy (WMS) and second-harmonic detection techniques were used. The modulation frequency and modulation depth were optimized theoretically and experimentally. Water vapor was added in the PAS sensor system to increase the vibrational-translational (V-T) relaxation rate of the CO molecule, which resulted in an ~8 times signal enhancement compared with the using of a dry CO/N2 gas mixture. The amplitude of the 2f signal had a 1.52-fold improvement compared to the one with only one time absorption. The experimental results showed that such a sensor had an excellent linear response to the optical power and gas concentration. At 1 s integration time, a minimum detection limit (MDL) for CO detection of 9.8 ppm was achieved. The long-term stability of the sensor system was evaluated with an Allan deviation analysis. When the integration time was 1100 s, the MDL improved to be 530 ppb. The detection performance of such a PAS-based CO sensor can be further improved when a laser with a higher output power and increasing optical absorption times is used.

Immersion photoacoustic spectrometer (iPAS) for arcing fault detection in power transformers.

Abstract: We report on the development of the first immersion photoacoustic spectrometer (iPAS) for arcing fault detection in power transformers. The spectrometer consists of a detection system and an all-optical photoacoustic sensing head mounted inside a small permeable chamber where dissolved C2H2 diffuses while the transformer oil is kept out. Our all-optical iPAS sensor can be placed directly inside an oil bath and measure dissolved C2H2 with the sensitivity and linearity needed for in situ arcing fault detection. Moreover, its fast response time holds great promise for extra-early fault diagnosis.

Cavity-Enhanced Raman and Helmholtz Resonator Photoacoustic Spectroscopy to Monitor the Mixed Sugar Metabolism of E. coli.

Abstract: We introduce and compare two powerful new techniques for headspace gas analysis above bacterial batch cultures by spectroscopy, Raman spectroscopy enhanced in an optical cavity (CERS), and photoacoustic detection in a differential Helmholtz resonator (DHR). Both techniques are able to monitor O2 and CO2 and its isotopomers with excellent sensitivity and time resolution to characterize bacterial growth and metabolism. We discuss and show some of the shortcomings of more conventional optical density (OD) measurements if used on their own without more sophisticated complementary measurements. The spectroscopic measurements can clearly and unambiguously distinguish the main phases of bacterial growth in the two media studied, LB and M9. We demonstrate how 13C isotopic labeling of sugars combined with spectroscopic detection allows the study of bacterial mixed sugar metabolism to establish whether sugars are sequentially or simultaneously metabolized. For E. coli, we have characterized the shift from glucose to lactose metabolism without a classic diauxic lag phase. DHR and CERS are shown to be cost-effective and highly selective analytical tools in the biosciences and in biotechnology, complementing and superseding existing conventional techniques. They also provide new capabilities for mechanistic investigations and show a great deal of promise for use in stable isotope bioassays.