Topic
Phthalic acid
About: Phthalic acid is a research topic. Over the lifetime, 3677 publications have been published within this topic receiving 50345 citations. The topic is also known as: o-benzenedicarboxylic acid & ortho-phthalic acid.
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TL;DR: A protocol for selective phosphopeptide enrichment using titanium dioxide (TiO2) chromatography is described, well suited for the characterization of phosphoproteins from both in vitro and in vivo studies in combination with mass spectrometry (MS).
Abstract: The characterization of phosphorylated proteins is a challenging analytical task since many of the proteins targeted for phosphorylation are low in abundance and phosphorylation is typically substoichiometric. Highly efficient enrichment procedures are therefore required. Here we describe a protocol for selective phosphopeptide enrichment using titanium dioxide (TiO2) chromatography. The selectivity toward phosphopeptides is obtained by loading the sample in a 2,5-dihydroxybenzoic acid (DHB) or phthalic acid solution containing acetonitrile and trifluoroacetic acid (TFA) onto a TiO2 micro-column. Although phosphopeptide enrichment can be achieved by using TFA and acetonitrile alone, the selectivity is dramatically enhanced by adding DHB or phthalic acid since these compounds, in conjunction with the low pH caused by TFA, prevent binding of nonphosphorylated peptides to TiO2. Using an alkaline solution (pH > or = 10.5) both monophosphorylated and multiphosphorylated peptides are eluted from the TiO2 beads. This highly efficient method for purification of phosphopeptides is well suited for the characterization of phosphoproteins from both in vitro and in vivo studies in combination with mass spectrometry (MS). It is a very easy and fast method. The entire protocol requires less than 15 min per sample if the buffers have been prepared in advance (not including lyophilization).
598 citations
TL;DR: In this paper, the specific adsorption of the aromatic acids, catechol, salicylic acid, benzoic acid, and phthalic acid on γ-Al2O3 is interpreted in terms of a ligand exchange model where anions of the organic acids replace the surface hydroxo groups of the Al2O 3 surface.
474 citations
TL;DR: Bi(2)WO(6) was successfully synthesized by a facile hydrothermal method, and characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and BET, showing mainly square-plate-like morphologies with a short edge and the average crystalline size was in the range of 50-150 nm.
372 citations
Patent•
22 Dec 2010TL;DR: Viscoelastic surfactant based aqueous fluid systems are useful as thickening agents in various applications, e.g. to suspend particles produced during the excavation of geologic formations as discussed by the authors.
Abstract: Viscoelastic surfactant based aqueous fluid systems useful as thickening agents in various applications, e.g. to suspend particles produced during the excavation of geologic formations. The surfactants are zwitterionic/amphoteric surfactants such as dihydroxyl alkyl glycinate, alkyl ampho acetate or propionate, alkyl betaine, alkyl amidopropyl betaine and alkylimino mono- or di-propionates derived from certain waxes, fats and oils. The thickening agent is used in conjunction with an inorganic water-soluble salt or organic additive such as phthalic acid, salicylic acid or their salts.
341 citations
TL;DR: It is shown that alizarin red and its products may be effectively degraded by the OH radicals produced by the reaction between the Fe(2+) ions and the electrogenerated H(2)O(2).
336 citations