Topic
Phytoalexin
About: Phytoalexin is a research topic. Over the lifetime, 1161 publications have been published within this topic receiving 63405 citations. The topic is also known as: phytoalexins.
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TL;DR: The results indicated STS was regulated in fruits depending on the developmental stage and significantly accumulation of STS mRNA and synthesis of new STS protein during the early of heat acclimation, which offers an important basis for further investigating the mechanism of post-harvest fruit adaptation to environmental stresses.
21 citations
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TL;DR: Induction of stress metabolites in the suspension cultured cells of eggplant (Solanum melongena L.) was examined and elicitation of these compounds was closely correlated with cellular damage in terms of the decrease of growth rate and was inhibited by 10 micromolar cycloheximide.
Abstract: Induction of stress metabolites in the suspension cultured cells of eggplant (Solanum melongena L.) was examined. When autoclaved RNase A or nigeran, both of which are nonspecific phytoalexin elicitors in bean cells, were added to the cell culture of eggplant, greatly enhanced levels of three compounds were observed. One of them was cis-pentadeca-6-ene-1,3-diyne-5,15-diol, a novel diacetylenic compound. This compound has considerable fungitoxic activity. Also identified was falcarindiol, another fungitoxic diacetylenic compound previously reported as one of the phytoalexins in infected tomato fruits and leaves. Elicited compounds preferentially accumulated in the culture medium rather than in the cells and decreased to original levels during prolonged culturing. The elicitation of these compounds was closely correlated with cellular damage in terms of the decrease of growth rate and was inhibited by 10 micromolar cycloheximide.
21 citations
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TL;DR: Testing a range of source tissues from soybean, in addition to chemical and biotic elicitors, to understand how to enhance the bioproduction of glyceollin I suggested that the biotic and chemical elicitors acted largely by separate mechanisms.
Abstract: Phytoalexins are metabolites biosynthesized in plants in response to pathogen, environmental, and chemical stresses that often have potent bioactivities, rendering them promising for use as therapeutics or scaffolds for pharmaceutical development. Glyceollin I is an isoflavonoid phytoalexin from soybean that exhibits potent anticancer activities and is not economical to synthesize. Here, we tested a range of source tissues from soybean, in addition to chemical and biotic elicitors, to understand how to enhance the bioproduction of glyceollin I. Combining the inorganic chemical silver nitrate (AgNO₃) with the wall glucan elicitor (WGE) from the soybean pathogen Phytophthora sojae had an additive effect on the elicitation of soybean seeds, resulting in a yield of up to 745.1 µg gt-1 glyceollin I. The additive elicitation suggested that the biotic and chemical elicitors acted largely by separate mechanisms. WGE caused a major accumulation of phytoalexin gene transcripts, whereas AgNO₃ inhibited and enhanced the degradation of glyceollin I and 6″-O-malonyldaidzin, respectively.
21 citations
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21 citations
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TL;DR: With both methods, higher inoculum dosages led to higher wilting rates and higher phytoalexin concentrations, and injection appears the most promising technique for the development of routine screening methods for resistance based on phy toaleXin accumulation.
Abstract: Five methods of stem inoculation of carnations with conidial suspensions ofFusarium oxysporum f.sp.dianthi were compared for uptake of the suspension, induction of phytoalexin accumulation and wilt development. Inoculation was performed by incision of the stem across droplets of inoculum placed on leaves, or by injection of droplets into the stem. With both methods, higher inoculum dosages led to higher wilting rates and higher phytoalexin concentrations. Injection was more effective than incision since a lower inoculum dosage was required to obtain the same phytoalexin levels. Injection therefore appears the most promising technique for the development of routine screening methods for resistance based on phytoalexin accumulation.
20 citations