Topic
Pichia pastoris
About: Pichia pastoris is a research topic. Over the lifetime, 7937 publications have been published within this topic receiving 162645 citations. The topic is also known as: Komagataella pastoris.
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TL;DR: Results show that culture parameters, such as the specific growth rate, may significantly affect the activity of glycoproteins produced in Pichia pastoris, and suggests that a compromise on thespecific growth rate may have to be found, in certain cases, to work with an acceptable productivity while avoiding the addition of many mannoses.
Abstract: A recombinant avidin-producing Mut(+) Pichia pastoris strain was used as a model organism to study the influence of the methanol feeding strategy on the specific product productivity (q(p)) and protein glycosylation. Fed-batch cultivations performed at various specific growth rates (A) and residual methanol concentrations showed that the specific avidin productivity is growth-dependent. The specific productivity increases strongly with the specific growth rate for mu ranging from 0 to 0.02 h(-1), and increases only slightly with the specific growth rate above this limit. N-terminal glycosylation was also found to be influenced by the specific growth rate, since 9-mannose glycans were the most abundant form at low growth rates, whereas 10-mannose carbohydrate. chains were favored at higher mu. These results show that culture parameters, such as the specific growth rate, may significantly affect the activity of glycoproteins produced in Pichia pastoris. In terms of process optimization, this suggests that a compromise on the specific growth rate may have to be found, in certain cases, to work with an acceptable productivity while avoiding the addition of many mannoses.
60 citations
TL;DR: Protein sequence analysis of the amino terminus showed that the correct processing to yield mature ghilanten varied with the fermentation conditions, and significant clonal variation in the expression of r-ghilanten was found among the His+ transformants.
60 citations
TL;DR: A recombinant form of hirudin (HIR), a potent thrombin inhibitor derived from the leech Hirudo medicinalis, was cloned and expressed in the methylotrophic yeast Pichia pastoris, offering an efficient system for production and purification of multigram quantities of biologically active rHIR for structure/function analyses.
60 citations
TL;DR: The neuritotrophic activity of APLP2 was compared to the APP isoforms sAPP695 and sAPP751 on chick sympathetic neurones and it was suggested that APP andAPLP2 have a similar or related role and supports the idea of a redundancy in function between the APP‐gene family proteins.
60 citations
TL;DR: A highly chloride tolerant laccase produced by the plant pathogen ascomycete Botrytis aclada is reported on, which was recombinantly expressed in Pichia pastoris with an extremely high yield and purified to homogeneity.
60 citations