Topic
Pichia pastoris
About: Pichia pastoris is a research topic. Over the lifetime, 7937 publications have been published within this topic receiving 162645 citations. The topic is also known as: Komagataella pastoris.
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TL;DR: A gene ( xyl11 ) encoding xylanase from Thermobifida fusca NTU22 was cloned, sequenced and expressed in Escherichia coli and secretively expressed in Pichia pastoris under the control of the AOX1 promoter.
55 citations
TL;DR: The purified enzyme showed highest activity between 50 and 70 °C at pH 7.0 and a preference for short-chain triglycerides (C4-C8), and significantly reduced activity was observed in the presence of hydrophilic esters.
Abstract: The current investigation focuses on shedding further light on the characteristics of lipase A from Candida antarctica (CalA), which has attracted growing attention in its suitability for industrial applications. CalA was functionally expressed in the methylotrophic yeast Pichia pastoris, purified and characterised. A classical fed-batch process and a semi-continuous process were developed and tested with regard to their yield capacity. Lipase concentrations of 0.88 and 0.55 g l(-1) were obtained using the fed-batch and semi-continuous processes, respectively. The semi-continuous process reaches a total activity of 10,233,000 U and so surpasses the fed-batch process reaching 7,530,000 U. The purified enzyme showed highest activity between 50 and 70 degrees C at pH 7.0 and a preference for short-chain triglycerides (C4-C8). Significantly reduced activity was observed in the presence of hydrophilic esters.
55 citations
TL;DR: Identification of proteinases, such as PEP2, which are apparently associated with the Aspergillus cell wall raises new interest in these molecules with respect to their possible function in the pathogenesis of invasive aspergillosis.
54 citations
TL;DR: The recombinant Pichia pastoris harboring the endoxylanase gene (TSEV1xyl) of Bacillus halodurans TSEV1 yielded a high titer of extracellular xylanase on induction with methanol which led to the purification of XOS as confirmed by TLC as well as HPLC analysis.
54 citations
TL;DR: The results show that P. pastoris derived HBsAg VLPs exhibit a high potential as a superior biosimilar vaccine against hepatitis B.
54 citations