Topic
Pichia pastoris
About: Pichia pastoris is a research topic. Over the lifetime, 7937 publications have been published within this topic receiving 162645 citations. The topic is also known as: Komagataella pastoris.
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TL;DR: Results from oral glucose tolerance test indicated that rEx‐4/HSA could effectively improve glucose tolerance in diabetic db/db mice and Pharmacokinetics studies in cynomologus monkeys showed that r exendin‐4‐HSA had a much longer plasma half‐life, which could potentially be used as a new recombinant biodrug for type 2 diabetes therapy.
Abstract: A novel recombinant exendin-4 human serum albumin fusion protein (rEx-4/HSA) expressed in Pichia pastoris was prepared and characterized. Ex-4 is a 39-amino acid peptide isolated from the salivary gland of the lizard Heloderma suspectum and is thought to be a novel therapeutic agent for type 2 diabetes. But to gain a continued effect, the peptide has to be injected twice a day owing to its short plasma half-life (t1/2 = 2.4 h). To extend the half-life of Ex-4 molecule in vivo, we designed a genetically engineered Ex-4/HSA fusion protein. Between Ex-4 and HSA, a peptide linker GGGGS was inserted and the fusion protein was expressed in methylotrophic yeast P. pastoris with native HSA secretion signal sequence. The recombinant protein was secreted correctly and was obtained with high purity (typically > 98%) by a three-step purification procedure. cAMP assay demonstrated that the fusion protein had a bioactivity similar to Ex-4 for interaction with GLP-1 receptors in vitro. Results from oral glucose tolerance test indicated that rEx-4/HSA could effectively improve glucose tolerance in diabetic db/db mice. Pharmacokinetics studies in cynomologus monkeys also showed that rEx-4/HSA had a much longer plasma half-life. Therefore, rEx-4/HSA fusion protein could potentially be used as a new recombinant biodrug for type 2 diabetes therapy. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.
49 citations
TL;DR: The recombinant anchor-less SAG1 proved competent for inducing proliferation, in vitro, of mononuclear cells from seropositive individuals and for inducing protection of mice against a lethal challenge with T. gondii tachyzoites.
49 citations
TL;DR: Ohta et al. as mentioned in this paper showed that Asp-73 and Glu-157 residues played a significant role in the xynI gene product's low pH optimum, and the N-terminal amino acid sequence and the apparent M r of the secreted recombinant protein indicated the native-like processing of the A. pullulans XynI signal sequence in Pichia pastoris.
49 citations
TL;DR: Results indicated that AiC 1qDC-2 could function as a pattern recognition receptor to recognize various PAMPs on different pathogens in the innate immune responses of scallop, and provided new clues to understand the role of invertebrate C1qDC proteins in the ancient complement system.
49 citations
TL;DR: In chemostat culture, dissolved oxygen concentration influenced product yield independently of any detectable influence on cell growth, and Glycerol was inhibitory to growth at high concentrations.
Abstract: Simple cyclic fed-batch culture (cfbc), consisting of a constant medium feed with periodic withdrawals of culture, resulted in a product yield (13.4 mg protein per gram biomass) similar to that obtained using the complex multiphase industrial production strategy (13.7 mg protein per gram biomass). In cfbc, productivity was ultimately limited by the rate at which the cells could assimilate methanol. Glycerol was inhibitory to growth at high concentrations. However, product yield continued to increase as the glycerol concentration was increased. In chemostat culture, dissolved oxygen concentration influenced product yield independently of any detectable influence on cell growth.
49 citations