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Pichia pastoris

About: Pichia pastoris is a research topic. Over the lifetime, 7937 publications have been published within this topic receiving 162645 citations. The topic is also known as: Komagataella pastoris.


Papers
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Journal ArticleDOI
TL;DR: A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system and the structure confirms the proposed existence of five disulfide bonds.
Abstract: A recombinant form of the sea raven type II antifreeze protein (SRAFP) has been produced using the Pichia pastoris expression system. The antifreeze activity of recombinant SRAFP is indistinguishable from that of the wild-type protein. The global fold of SRAFP has been determined by two-dimensional 1H homonuclear and three-dimensional 1H-?15N? heteronuclear NMR spectroscopy using 785 NOE distance restraints and 47 angular restraints. The molecule folds into one globular domain that consists of two helices and nine beta-strands in two beta-sheets. The structure confirms the proposed existence of five disulfide bonds. The global fold of SRAFP is homologous to C-type lectins and pancreatic stone proteins, even though the sequence identity is only approximately 20%.

125 citations

Journal ArticleDOI
TL;DR: A simple and inexpensive methanol control system will help bioengineering studies on the production of recombinant proteins in P. pastoris, the growth and production of objective proteins in which the energy for the production competed with that for cell growth.

125 citations

Journal ArticleDOI
TL;DR: The expression of a humanized single-chain variable domain fragment antibody (A33scFv) was optimized for Pichia pastoris with yields exceeding 4 g L(-1) and demonstrates that functional A33 scFv can be produced in sufficient quantities using P. pastoris.

124 citations

Journal ArticleDOI
TL;DR: The fusion of the CBD to the lipase resulted in a greatly enhanced binding toward cellulose for CBD-CALB compared with that for CALB, and results from endoglycosidase H digestion of the proteins showed that CALB and CBD-cALB were N-glycosylated.

124 citations

Journal ArticleDOI
TL;DR: The synergistic activity exhibited by StCel61a and its dependence on reducing substances provide guidelines for process design towards the production of economically viable bioethanol.

124 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023150
2022340
2021255
2020303
2019374
2018401