Topic
Pichia pastoris
About: Pichia pastoris is a research topic. Over the lifetime, 7937 publications have been published within this topic receiving 162645 citations. The topic is also known as: Komagataella pastoris.
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TL;DR: The cloning of the gene coding for this lipase/acyltransferase in the yeast Pichia pastoris is described and the heterologous high-level expression of the recombinant enzyme is described.
86 citations
TL;DR: Carbon source metabolism and the central carbon pathways of P. pastoris metabolism are reviewed to enable fine-tuning bioreactor operation parameters in relation with the physiology of the microorganism in order to develop new strategies for the enhanced r-protein production.
86 citations
TL;DR: Pichia pastoris produced in high yields recombinant alpha-amylase that is similar with respect to structure and function to the enzyme purified from malt extracts, which greatly facilitates future mutational analysis of barley alpha- amylase in order to probe structure/function relationships.
86 citations
TL;DR: Aspergillus niger xylanase is the first microbial xylan enzyme efficiently secreted and correctly processed by P. pastoris, and follows normal Michaelis–Menten kinetics with K m and V max values similar for both enzymes.
86 citations
TL;DR: A recombinant Hc fragment of botulinum neurotoxin, serotype B (rBoNTB(Hc), has been successfully expressed in a Mut+ strain of the methylotrophic yeast Pichia pastoris for use as an antigen in a proposed human vaccine.
86 citations